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Literature summary extracted from

  • Agius, F.; Gonzalez-Lamothe, R.; Caballero, J.L.; Munoz-Blanco, J.; Botella, M.A.; Valpuesta, V.
    Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase (2003), Nat. Biotechnol., 21, 177-181.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.365 expressed in Arabidopsis thaliana and in Escherichia coli XL1-Blue MRF' cells Fragaria x ananassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+ Fragaria x ananassa
-
 L-galactonate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.365 Fragaria x ananassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.365 glutathione agarose column chromatography Fragaria x ananassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.365 fruit
-
 Fragaria x ananassa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+
-
 Fragaria x ananassa L-galactonate + NADP+
-
 ?
1.1.1.365 D-glucuronate + NADPH + H+
-
 Fragaria x ananassa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.365 AKR2
-
 Fragaria x ananassa
1.1.1.365 D-galacturonic acid reductase
-
 Fragaria x ananassa
1.1.1.365 GalUR
-
 Fragaria x ananassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.365 NADPH dependent on Fragaria x ananassa

General Information

EC Number General Information Comment Organism
1.1.1.365 physiological function enzyme expression correlates with changing ascorbic acid content in strawberry fruit during ripening. Overexpression of the enzyme in Arabidopsis thaliana enhances vitamin C content 2-3fold Fragaria x ananassa