Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kuntze, K.; Kiefer, P.; Baumann, S.; Seifert, J.; von Bergen, M.; Vorholt, J.; Boll, M.
    Enzymes involved in the anaerobic degradation of meta-substituted halobenzoates (2011), Mol. Microbiol., 82, 758-769.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.7.8 Ti(III)-citrate required, reductive dehalogenation of 3-chlorobenzoyl-CoA is oxygen sensitive and strictly dependent on the presence of ATP and Ti(III)-citrate Thauera chlorobenzoica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.7.8 O2 reductive dehalogenation of 3-chlorobenzoyl-CoA is oxygen sensitive and strictly dependent on the presence of ATP and Ti(III)-citrate Thauera chlorobenzoica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.7.8 Mg2+ required, reductive dehalogenation of 3-chlorobenzoyl-CoA is oxygen sensitive and strictly dependent on the presence of ATP and Ti(III)-citrate Thauera chlorobenzoica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.8 3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Thauera chlorobenzoica
-
 3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Thauera chlorobenzoica 3CB-1
-
 3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Thauera chlorobenzoica
-
 3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Thauera chlorobenzoica 3CB-1
-
 3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Thauera chlorobenzoica
-
 3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Thauera chlorobenzoica 3CB-1
-
 3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Thauera chlorobenzoica
-
 cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 additional information Thauera chlorobenzoica the enzyme catalyzes ATP-dependent reductive dehalogenation of 3-chloro/3-bromobenzoyl-CoA to benzoyl-CoA, whereas 3-fluorobenzoyl-CoA is converted to a fluorinated cyclic dienoyl-CoA compound. Reductive dechlorination/debromination of 3-Cl-/3-Br-benzoyl-CoA catalyzed by class I BCR yielding benzoyl-CoA, the latter is subsequently dearomatized by the same enzyme. The elimination of HCl/HBr may occur enzymatically or spontaneously ?
-
 ?
1.3.7.8 additional information Thauera chlorobenzoica 3CB-1 the enzyme catalyzes ATP-dependent reductive dehalogenation of 3-chloro/3-bromobenzoyl-CoA to benzoyl-CoA, whereas 3-fluorobenzoyl-CoA is converted to a fluorinated cyclic dienoyl-CoA compound. Reductive dechlorination/debromination of 3-Cl-/3-Br-benzoyl-CoA catalyzed by class I BCR yielding benzoyl-CoA, the latter is subsequently dearomatized by the same enzyme. The elimination of HCl/HBr may occur enzymatically or spontaneously ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.8 Thauera chlorobenzoica
-
-
-
1.3.7.8 Thauera chlorobenzoica 3CB-1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.7.8 0.022
-
 specific activtity with 3-bromo-halogenated benzoyl-CoA as substrate with extracts from cells grown on benzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.022
-
 specific activtity with 3-chloro-halogenated benzoyl-CoA as substrate with extracts from cells grown on 3-bromobenzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.025
-
 specific activtity with 3-bromo-halogenated benzoyl-CoA as substrate with extracts from cells grown on 3-chlorobenzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.025
-
 specific activtity with 3-chloro-halogenated benzoyl-CoA as substrate with extracts from cells grown on benzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.0275
-
 specific activtity with 3-bromo-halogenated benzoyl-CoA as substrate with extracts from cells grown on 3-bromobenzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.0275
-
 specific activtity with benzoyl-CoA as substrate with extracts from cells grown on 3-chlorobenzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.0335
-
 specific activtity with 3-chloro-halogenated benzoyl-CoA as substrate with extracts from cells grown on 3-chlorobenzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.041
-
 specific activtity with benzoyl-CoA as substrate with extracts from cells grown on 3-bromobenzoate, pH 7.3, 30°C Thauera chlorobenzoica
1.3.7.8 0.045
-
 specific activtity with benzoyl-CoA as substrate with extracts from cells grown on benzoate, pH 7.3, 30°C Thauera chlorobenzoica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.8 3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
-
 Thauera chlorobenzoica 3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-bromobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
-
 Thauera chlorobenzoica 3CB-1 3-bromocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
-
 Thauera chlorobenzoica 3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
-
 Thauera chlorobenzoica 3CB-1 3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
-
 Thauera chlorobenzoica 3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O 3-fluorobenzoyl-CoA is converted only to the fluorinated cyclic dienoyl-CoA compound Thauera chlorobenzoica 3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
-
 Thauera chlorobenzoica 3CB-1 3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
-
 Thauera chlorobenzoica cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
1.3.7.8 additional information the enzyme catalyzes ATP-dependent reductive dehalogenation of 3-chloro/3-bromobenzoyl-CoA to benzoyl-CoA, whereas 3-fluorobenzoyl-CoA is converted to a fluorinated cyclic dienoyl-CoA compound. Reductive dechlorination/debromination of 3-Cl-/3-Br-benzoyl-CoA catalyzed by class I BCR yielding benzoyl-CoA, the latter is subsequently dearomatized by the same enzyme. The elimination of HCl/HBr may occur enzymatically or spontaneously Thauera chlorobenzoica ?
-
 ?
1.3.7.8 additional information reactions under anaerobic conditions Thauera chlorobenzoica ?
-
 ?
1.3.7.8 additional information the enzyme catalyzes ATP-dependent reductive dehalogenation of 3-chloro/3-bromobenzoyl-CoA to benzoyl-CoA, whereas 3-fluorobenzoyl-CoA is converted to a fluorinated cyclic dienoyl-CoA compound. Reductive dechlorination/debromination of 3-Cl-/3-Br-benzoyl-CoA catalyzed by class I BCR yielding benzoyl-CoA, the latter is subsequently dearomatized by the same enzyme. The elimination of HCl/HBr may occur enzymatically or spontaneously Thauera chlorobenzoica 3CB-1 ?
-
 ?
1.3.7.8 additional information reactions under anaerobic conditions Thauera chlorobenzoica 3CB-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.8 ATP-dependent benzoyl-CoA reductase (class I)
-
 Thauera chlorobenzoica
1.3.7.8 ATP-dependent class I benzoyl-CoA reductase
-
 Thauera chlorobenzoica
1.3.7.8 BCR
-
 Thauera chlorobenzoica
1.3.7.8 class I BCR
-
 Thauera chlorobenzoica
1.3.7.8 halobenzoyl-CoA reductase
-
 Thauera chlorobenzoica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.7.8 30
-
 assay at Thauera chlorobenzoica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.7.8 7.3
-
 assay at Thauera chlorobenzoica

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.8 ATP
-
 Thauera chlorobenzoica
1.3.7.8 Ferredoxin
-
 Thauera chlorobenzoica

Expression

EC Number Organism Comment Expression
1.3.7.8 Thauera chlorobenzoica reductive dehalogenase activity is not specifically induced by the halogenated aromatic growth substrates additional information

General Information

EC Number General Information Comment Organism
1.3.7.8 metabolism proposed role of class I benzoyl-CoA reductase in the metabolism of halobenzoates, overview Thauera chlorobenzoica
1.3.7.8 additional information 3-chloro/3-bromobenzoyl-CoA dehalogenation/elimination activity is equally present in extracts from Thauera chlorobenzoica grown on 3-chlorobenzoate and benzoate suggesting that no 3-Cl-benzoate-specific class I BCR is induced Thauera chlorobenzoica