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Literature summary extracted from
- Functional divergence and catalytic properties of dehydroascorbate reductase family proteins from Populus tomentosa (2013), Mol. Biol. Rep., 40, 5105-5114.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | expression in Escherichia coli | Populus tomentosa |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | modeling of structure. Protein has a typical glutathione S-transferase structure containing a smaller thioredoxin-like N-terminal domain and a larger helical C-terminal domain | Populus tomentosa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | D72A | reduction in catalytic efficincy. D72 is a glutathione-site residue | Populus tomentosa |
| 1.8.5.1 | K8A | severe reduction in catalytic efficincy. K8 is a glutathione-site residue | Populus tomentosa |
| 1.8.5.1 | S73A | reduction in catalytic efficincy. S73 is a glutathione-site residue | Populus tomentosa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 0.07 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa |  |
| 1.8.5.1 | 0.13 | - |
dehydroascorbate | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 0.23 | - |
dehydroascorbate | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 0.48 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 0.7 | - |
dehydroascorbate | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 0.97 | - |
dehydroascorbate | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 1.71 | - |
glutathione | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 2.28 | - |
glutathione | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 2.47 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 3.01 | - |
glutathione | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 3.26 | - |
glutathione | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 3.7 | 5 | glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.5.1 | chloroplast | - |
Populus tomentosa | 9507 | - |
| 1.8.5.1 | cytosol | - |
Populus tomentosa | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 29000 | 40000 | gel filtration | Populus tomentosa |
| 1.8.5.1 | 29100 | - |
1 * 29100, calculated, about 2900, MALDI-TOF | Populus tomentosa |
| 1.8.5.1 | 29700 | - |
1 * 29700, calculated, about 2900, MALDI-TOF | Populus tomentosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.1 | Populus tomentosa | J9WN12 | isoform DHAR2, c.f. EC 2.5.1.18 | - |
| 1.8.5.1 | Populus tomentosa | J9WNR5 | isoform DHAR3, c.f. EC 2.5.1.18 | - |
| 1.8.5.1 | Populus tomentosa | J9WQY6 | isoform DHAR1, c.f. EC 2.5.1.18 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 53 | - |
pH not specified in the publication, temperature not specified in the publication | Populus tomentosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | 2 glutathione + dehydroascorbate | - |
Populus tomentosa | glutathione disulfide + ascorbate | - |
? | |
| 1.8.5.1 | additional information | no substrates: 1-chloro-2,4-dinitrobenzene, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, nitrobutyl chloride, 4-nitrophenyl acetate, 1,2-dichloro-4-nitrobenzene | Populus tomentosa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | monomer | 1 * 29100, calculated, about 2900, MALDI-TOF | Populus tomentosa |
| 1.8.5.1 | monomer | 1 * 29700, calculated, about 2900, MALDI-TOF | Populus tomentosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | DHAR1 | - |
Populus tomentosa |
| 1.8.5.1 | DHAR2 | - |
Populus tomentosa |
| 1.8.5.1 | DHAR3 | - |
Populus tomentosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 30 | 35 | - |
Populus tomentosa |
| 1.8.5.1 | 45 | - |
- |
Populus tomentosa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 15 | - |
66% of maximum activity | Populus tomentosa |
| 1.8.5.1 | 55 | - |
51% of maximum activity | Populus tomentosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 570 | - |
dehydroascorbate | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 745 | - |
glutathione | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 3988 | - |
dehydroascorbate | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 5737 | - |
dehydroascorbate | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 6705 | - |
glutathione | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 13130 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 15960 | - |
glutathione | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 17730 | - |
dehydroascorbate | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 21150 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 40850 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 53880 | - |
glutathione | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 99830 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 7.5 | - |
- |
Populus tomentosa |
| 1.8.5.1 | 8 | - |
- |
Populus tomentosa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 6.5 | 8.5 | more than 66% of maximum activity | Populus tomentosa |
| 1.8.5.1 | 6.5 | - |
41% of maximum activity | Populus tomentosa |
| 1.8.5.1 | 8.5 | - |
86% of maximum activity | Populus tomentosa |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.1 | 247 | - |
glutathione | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 588 | - |
dehydroascorbate | mutant K8A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 2056 | - |
glutathione | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 5697 | - |
dehydroascorbate | mutant S73A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 7708 | - |
dehydroascorbate | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 9332 | - |
glutathione | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 16540 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 18760 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 23630 | - |
glutathione | wild-type, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 26620 | - |
glutathione | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 44050 | - |
dehydroascorbate | pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
| 1.8.5.1 | 44130 | - |
dehydroascorbate | mutant D72A, pH not specified in the publication, temperature not specified in the publication | Populus tomentosa | |
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