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Literature summary extracted from

  • Li, S.; Li, X.; Zhao, H.; Cai, B.
    Physiological role of the novel salicylaldehyde dehydrogenase NahV in mineralization of naphthalene by Pseudomonas putida ND6 (2011), Microbiol. Res., 166, 643-653.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.65 Pseudomonas putida
-
-
-
1.2.1.65 Pseudomonas putida ND6
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-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.65 15
-
nahF mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C Pseudomonas putida
1.2.1.65 67.81
-
nahV mutant with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C Pseudomonas putida
1.2.1.65 85.4
-
wild-type ND6 with naphthalene as the sole carbon and energy source, pH 7.4 and 25°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.65 additional information nahV mRNA levels are 3fold lower than the nahF mRNA levels in the wild-type ND6 strain Pseudomonas putida ?
-
?
1.2.1.65 additional information nahV mRNA levels are 3fold lower than the nahF mRNA levels in the wild-type ND6 strain Pseudomonas putida ND6 ?
-
?
1.2.1.65 salicylaldehyde + NAD+ + H2O conversion of salicylaldehyde to salicylate in Pseudomonas putida ND6 is mediated by two isofunctional, inducible, plasmid-encoded enzymes, NahF and NahV Pseudomonas putida salicylate + NADH + 2 H+
-
?
1.2.1.65 salicylaldehyde + NAD+ + H2O conversion of salicylaldehyde to salicylate in Pseudomonas putida ND6 is mediated by two isofunctional, inducible, plasmid-encoded enzymes, NahF and NahV Pseudomonas putida ND6 salicylate + NADH + 2 H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.65 NahF
-
Pseudomonas putida
1.2.1.65 NahV
-
Pseudomonas putida
1.2.1.65 salicylaldehyde dehydrogenase
-
Pseudomonas putida

Expression

EC Number Organism Comment Expression
1.2.1.65 Pseudomonas putida a 10fold increase in nahV expression observed in the ND6-NahF mutant when cultured in the presence of naphthalene and a 30fold increase when cultured in the presence of salicylate up
1.2.1.65 Pseudomonas putida nahF expression levels in the ND6-NahV mutant elevated by 20fold compared to the levels observed in the wild-type strain cultured in the presence of naphthalene and a 5fold increase when cultured in the presence of salicylate up

General Information

EC Number General Information Comment Organism
1.2.1.65 metabolism mineralization of naphthalene, detoxification of salicylaldehyde Pseudomonas putida