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Literature summary extracted from

  • Sygmund, C.; Staudigl, P.; Klausberger, M.; Pinotsis, N.; Djinovic-Carugo, K.; Gorton, L.; Haltrich, D.; Ludwig, R.
    Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris (2011), Microb. Cell Fact., 10, 106.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 recombinant overexpression in Escherichia coli strains Rosetta 2, T7 Express and T7 Express (pGro7) and Pichia pastoris strain X-33, with a much higher expression level and 4800fold higher enzyme activity in Pichia pastoris, fed-batch cultivation of a Pichia pastoris transformant, method evaluation, overview Colletotrichum gloeosporioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.9 2 3 D-xylose recombinant enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 10.1
-
D-glucose recombinant enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 10.2
-
D-glucose native enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 17.1
-
D-glucose recombinant enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 19
-
D-glucose native enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 21
-
D-xylose native enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 24
-
D-xylose native enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 26
-
D-xylose recombinant enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.9 67000
-
x * 67000, deglycosylated recombinant enzyme expressed from Pichia pastoris, SDS-PAGE Colletotrichum gloeosporioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.9 D-glucose + a quinone Colletotrichum gloeosporioides
-
D-glucono-1,5-lactone + a quinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Colletotrichum gloeosporioides
-
anamorph Colletotrichum gloeosporoides
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.5.9 glycoprotein
-
Colletotrichum gloeosporioides

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 recombinant enzyme from Pichia pastoris strain X-33 5.1fold by hydrophobic interaction and anion exchange chromatography Colletotrichum gloeosporioides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.9 836
-
purified recombinant enzyme expressed from Pichia pastoris, pH 7.5, 30°C Colletotrichum gloeosporioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + a quinone
-
Colletotrichum gloeosporioides D-glucono-1,5-lactone + a quinol
-
?
1.1.5.9 D-glucose + ferricenium ion
-
Colletotrichum gloeosporioides D-glucono-1,5-lactone + ferrocenium ion
-
?
1.1.5.9 D-xylose + ferricenium ion
-
Colletotrichum gloeosporioides D-xylono-1,5-lactone + ferrocenium ion
-
?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 ? x * 67000, deglycosylated recombinant enzyme expressed from Pichia pastoris, SDS-PAGE Colletotrichum gloeosporioides

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 D-glucose:acceptor 1-oxidoreductase
-
Colletotrichum gloeosporioides
1.1.5.9 FAD-dependent glucose dehydrogenase
-
Colletotrichum gloeosporioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.9 46
-
recombinant enzyme expressed from Pichia pastoris Colletotrichum gloeosporioides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.9 56
-
pH-dependent thermal stability with the highest Tm values in the acidic range of pH 4.5 to pH 6.4. The maximum Tm value of 56°C is measured in 50 mM sodium acetate buffer pH 5.0 and in 50 mM MES buffer pH 5.8 Colletotrichum gloeosporioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.9 40
-
D-xylose native enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 53
-
D-xylose recombinant enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 60
-
D-xylose native enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 61
-
D-xylose recombinant enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 179
-
D-glucose recombinant enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 180
-
D-glucose native enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 380
-
D-glucose native enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 418
-
D-glucose recombinant enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.9 7.5
-
recombinant enzyme Colletotrichum gloeosporioides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.5.9 5 5.8 pH-dependent thermal stability with the highest Tm values in the acidic range of pH 4.5 to pH 6.4. The maximum Tm value of 56°C is measured in 50 mM sodium acetate buffer pH 5.0 and in 50 mM MES buffer pH 5.8 Colletotrichum gloeosporioides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD dependent on Colletotrichum gloeosporioides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.5.9 1.9
-
D-xylose native enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 2
-
D-xylose recombinant enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 2.5
-
D-xylose native enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 2.7
-
D-xylose recombinant enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 17.6
-
D-glucose native enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 17.7
-
D-glucose recombinant enzyme, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 20
-
D-glucose native enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 24.5
-
D-glucose recombinant enzyme, pH 7.5, 30°C Colletotrichum gloeosporioides