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Literature summary extracted from

  • Dang, M.; Pochapsky, S.; Pochapsky, T.
    Spring-loading the active site of cytochrome P450 cam (2011), Metallomics, 3, 339-343.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 G326A site-directed mutagenesis in order to decrease the flexibility of the polypeptide at that point, spin state fractions with different substrates and compared to the wild-type enzyme. The mutant shows 40% reduced activity compared to the wild-type enzyme Pseudomonas putida
1.14.15.1 additional information generation of a G327 insertion mutant in order to determine whether the length of the helix played a role in the sensitivity of the K' helix to substrate, the insertion mutant fails to express Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Fe2+ heme and cytochrome P450 containing enzyme Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2 Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.1 cell culture Pseudomonas putida strains can use (1R)-(+) camphor as sole carbon and energy source Pseudomonas putida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 adamantenone + reduced putidaredoxin + O2
-
 Pseudomonas putida ?
-
 ?
1.14.15.1 norcamphor + reduced putidaredoxin + O2
-
 Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 CYP101A1
-
 Pseudomonas putida
1.14.15.1 cytochrome P450 cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome P450
-
 Pseudomonas putida
1.14.15.1 heme
-
 Pseudomonas putida
1.14.15.1 putidaredoxin
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.15.1 evolution the enzyme belongs to the superfamily of cytochrome P450 monooxygenases Pseudomonas putida
1.14.15.1 additional information substrate recognition and selectivity, enzyme-substrate interactions, NMR structrue analysis, Analysis of 1H,15N-TROSY-HSQC spectra of CYP-(+)-camphor-CO, CYP-adamantenone-CO and CYP-norcamphor-CO. overview. Replacing the native substrate camphor with adamantanone or norcamphor causes perturbations in NMR-detected NH correlations assigned to the network,which includes portions of a beta-sheet and an adjacent helix that is remote from the active site Pseudomonas putida