EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.5.4 | expression in Escherichia coli | Acidithiobacillus ferrooxidans |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.5.4 | crystal structures of the wild-type enzyme in complex with sodium selenide and gold(I) cyanide. Mechanism for the reduction of sulfides to elemental sulfur may involve nucleophilic attack of Cys356 on C4A atom of FAD or alternatively, an alternative anionic radical mechanism by direct electron transfer from Cys356 to the isoalloxazine ring of FAD | Acidithiobacillus ferrooxidans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.5.4 | C128A | about 35% of wild-type activity in assay with decylubiquinone | Acidithiobacillus ferrooxidans |
1.8.5.4 | C128S | loss of activity in assay with decylubiquinone | Acidithiobacillus ferrooxidans |
1.8.5.4 | C160A | loss of activity in assay with decylubiquinone, about 35% of wild-type activity for reduction of FAD fluorescence by Na2S | Acidithiobacillus ferrooxidans |
1.8.5.4 | C356S | loss of activity in assay with decylubiquinone, loss of activity for reduction of FAD fluorescence by Na2S | Acidithiobacillus ferrooxidans |
1.8.5.4 | H132A | about 40% of wild-type activity in assay with decylubiquinone | Acidithiobacillus ferrooxidans |
1.8.5.4 | H198A | about 60% of wild-type activity in assay with decylubiquinone | Acidithiobacillus ferrooxidans |
1.8.5.4 | S126A | about 35% of wild-type activity in assay with decylubiquinone | Acidithiobacillus ferrooxidans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.5.4 | Acidithiobacillus ferrooxidans | B7JBP8 | - |
- |
1.8.5.4 | Acidithiobacillus ferrooxidans DSM 14882 | B7JBP8 | - |
- |