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Literature summary extracted from

  • Seehuber, A.; Dahint, R.
    Conformation and activity of glucose oxidase on homogeneously coated and nanostructured surfaces (2013), J. Phys. Chem. B, 117, 6980-6989.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 analysis the enzyme is useful as biosensor for glucose detection Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.4 additional information enzyme adsorption on different particles with homogeneous or nanostructured surfaces and coated with different compounds, i.e. 11-amino-1-undecanethiol, 12-mercaptododecanoic acid, 1-dodecanethiol, and 11-(1H-pyrol-11-(1H-pyrol-1-yl)undecane-1-thiol), only 9% of the activity of the native protein is preserved on 11-(1H-pyrol-11-(1H-pyrol-1-yl)undecane-1-thiol), but the substrate affinity of the adsorbed GOx is best on 11-(1H-pyrol-11-(1H-pyrol-1-yl)undecane-1-thiol) where its catalytic activity is worst, secondary structure of thhe enzyme is altered compared to enzyme in solution, overview Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 additional information
-
 additional information Michaelis-Menten kinetics Aspergillus niger
1.1.3.4 0.019
-
 beta-D-glucose native enzyme in solution, pH 5.5, temperature not specified in the publication Aspergillus niger
1.1.3.4 8
-
 beta-D-glucose enzyme adsorbed on 11-(1H-pyrol-11-(1H-pyrol-1-yl)undecane-1-thiol) coated matrix, high enzyme concentration, pH 5.5, temperature not specified in the publication Aspergillus niger
1.1.3.4 12
-
 beta-D-glucose enzyme adsorbed on 11-amino-1-undecanethiol coated matrix, pH 5.5, temperature not specified in the publication Aspergillus niger
1.1.3.4 22
-
 beta-D-glucose enzyme adsorbed on 11-(1H-pyrol-11-(1H-pyrol-1-yl)undecane-1-thiol) coated matrix, low enzyme concentration, pH 5.5, temperature not specified in the publication Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.4 soluble
-
 Aspergillus niger
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 commercial preparation
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.4 100
-
 immobilized enzyme, pH 5.5, temperature not specified in the publication, high enzyme concentration Aspergillus niger
1.1.3.4 216
-
 immobilized enzyme, pH 5.5, temperature not specified in the publication, low enzyme concentration Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 beta-D-glucose + O2
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.4 GOX
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.5
-
 assay at Aspergillus niger