EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.4 | diethyldithiocarbamate | 35% increase of activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | DW 1750000 | 21% increase of activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | additional information | compared to free enzyme, MnO2 nanoparticles(NP)-bound enzyme shows improved activity (35% stimulation at 2.5 mg/ml) while ZnO NPs- and CuO NPs-bound enzymes have no substantial improvement | Hordeum vulgare |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.4 | Ca2+ | 92% residual activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | K+ | 94% residual activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | additional information | not influenced by EDTA, NaCl, and MgSO4 | Hordeum vulgare | |
1.2.3.4 | NEM | 92% residual activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | Sodium azide | 86% residual activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | Sodium molybdate | 71% residual activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | Sodium nitrate | 45% residual activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | sodium thiocyanate | 72% residual activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | ZnSO4 | 90% residual activity at 1 mM | Hordeum vulgare |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.4 | Cu2+ | 21% increase of activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | Fe2+ | 10% increase of activity at 1 mM | Hordeum vulgare | |
1.2.3.4 | Mn2+ | 37% increase of activity at 1 mM | Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.4 | oxalate + O2 + H+ | Hordeum vulgare | - |
CO2 + H2O2 | - |
? | |
1.2.3.4 | oxalate + O2 + H+ | Hordeum vulgare BH393 | - |
CO2 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.3.4 | Hordeum vulgare | - |
- |
- |
1.2.3.4 | Hordeum vulgare BH393 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.3.4 | DEAE-Sephacel column chromatography and Sephadex G-100 gel filtration | Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.3.4 | root | - |
Hordeum vulgare | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.3.4 | 4-8°C, 90 days, native enzyme, up to 70% loss of activity | Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.4 | oxalate + O2 + H+ | - |
Hordeum vulgare | CO2 + H2O2 | - |
? | |
1.2.3.4 | oxalate + O2 + H+ | - |
Hordeum vulgare BH393 | CO2 + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.3.4 | oxalate: O2 oxidoreductase | - |
Hordeum vulgare |
1.2.3.4 | OXO | - |
Hordeum vulgare |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.3.4 | 35 | - |
nanoparticle-bound enzyme | Hordeum vulgare |
1.2.3.4 | 40 | - |
free enzyme | Hordeum vulgare |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.3.4 | 70 | - |
metal oxide nanoparticles(NP)-bound enzyme retains more activity when subjected to thermal treatment at 70°C for 30 min., retention of activity in the increasing order being 54%, 65%, 76%, and 87% for native, ZnO NPs-, CuO NPs-, and MnO2 NPs-bound enzyme, respectively | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.3.4 | 3.2 | - |
free enzyme | Hordeum vulgare |
1.2.3.4 | 4 | - |
nanoparticle-bound enzyme | Hordeum vulgare |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.4 | additional information | FMN, FAD, NAD+, and riboflavin at 1 mM in the presence of nanoparticles have practically no effect on native and immobilized enzymes | Hordeum vulgare |