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Literature summary extracted from

  • Rosini, E.; Nossa, S.; Valentino, M.; DArrigo, P.; Marinesco, S.; Pollegioni, L.
    Expression of rat diamine oxidase in Escherichia coli (2012), J. Mol. Catal. B, 82, 115-120.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.22 expressed in Escherichia coli Origami2(DE3) cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.22 histamine substrate inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.035
-
 histamine in 100 mM potassium phosphate, pH 7.2, at 37°C Rattus norvegicus
1.4.3.22 0.45
-
 tyramine in 100 mM potassium phosphate, pH 7.2, at 37°C Rattus norvegicus
1.4.3.22 0.64
-
 benzylamine in 100 mM potassium phosphate, pH 7.2, at 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.22 Cu2+ Cu2+ ions stimulate the activation/maturation of the enzyme. The highest activity is observed at more than 5fold molar ratio between Cu2+ and the enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 80000
-
 x * 80000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.22 histamine + H2O + O2 Rattus norvegicus preferred substrate (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22 Ni-NTA column chromatography and Superdex 200 gel filtration Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 benzylamine + H2O + O2
-
 Rattus norvegicus benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.22 histamine + H2O + O2 preferred substrate Rattus norvegicus (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.22 phenylethylamine + H2O + O2
-
 Rattus norvegicus ?
-
 ?
1.4.3.22 tyramine + H2O + O2
-
 Rattus norvegicus (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 ? x * 80000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.22 DAO
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.22 30
-
 histamine pH and temperature not specified in the publication Rattus norvegicus