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Literature summary extracted from

  • Fincato, P.; Moschou, P.N.; Spedaletti, V.; Tavazza, R.; Angelini, R.; Federico, R.; Roubelakis-Angelakis, K.A.; Tavladoraki, P.
    Functional diversity inside the Arabidopsis polyamine oxidase gene family (2011), J. Exp. Bot., 62, 1155-1168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.14 ZmPAO, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His-tagged enzyme in Pichia pastoris Zea mays
1.5.3.16 AtPAO1, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method Arabidopsis thaliana
1.5.3.17 AtPAO2, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method Arabidopsis thaliana
1.5.3.17 AtPAO3, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, recombinant expression as maltose-binding protein fusion protein in Escherichia coli, and expression of GFP-tagged AtPAO3 in transgenic Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method resulting in increased putrescine levels after feeding of exogenous spermidine Arabidopsis thaliana
1.5.3.17 AtPAO4, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.17 additional information homozygous lines of T-DNA insertional mutant of Arabidopsis thaliana SALK_121288 for the AtPAO3 gene Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.16 guazatine
-
Arabidopsis thaliana
1.5.3.17 guazatine
-
Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.14 0.0016
-
spermine pH 7.5, 25°C, recombinant ZmPAO Zea mays
1.5.3.16 additional information
-
additional information Michaelis-Menten kinetics Arabidopsis thaliana
1.5.3.16 0.12
-
spermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.16 0.47
-
N1-acetylspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.16 5.7
-
thermospermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.16 6.9
-
norspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.17 additional information
-
additional information Michaelis-Menten kinetics Arabidopsis thaliana
1.5.3.17 0.045
-
norspermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.047
-
spermine pH 7.5, 25°C, recombinant AtPAO4 Arabidopsis thaliana
1.5.3.17 0.05
-
thermospermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.139
-
spermidine pH 7.5, 25°C, recombinant AtPAO4 Arabidopsis thaliana
1.5.3.17 0.233
-
N1-acetylspermine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 0.27
-
spermine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 0.274
-
spermidine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.409
-
spermidine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 0.58
-
spermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 2
-
N1-acetylspermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.3.17 peroxisome
-
Arabidopsis thaliana 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.3.17 additional information AtPAO4 is rapidly inactivated under high ionic strength conditions Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.14 spermidine + O2 + H2O Zea mays
-
propane-1,3-diamine + 4-aminobutanal + H2O2
-
?
1.5.3.14 spermine + O2 + H2O Zea mays
-
propane-1,3-diamine + N-(3-aminopropyl)-4-aminobutanal + H2O2
-
?
1.5.3.16 spermine + O2 + H2O Arabidopsis thaliana
-
spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.17 N1-acetylspermidine + O2 + H2O Arabidopsis thaliana
-
putrescine + 3-acetamidopropanal + H2O2
-
?
1.5.3.17 N1-acetylspermidine + O2 + H2O Arabidopsis thaliana Columbia-0
-
putrescine + 3-acetamidopropanal + H2O2
-
?
1.5.3.17 N1-acetylspermine + O2 + H2O Arabidopsis thaliana
-
spermidine + N-acetyl-3-aminopropanal + H2O2
-
?
1.5.3.17 N1-acetylspermine + O2 + H2O Arabidopsis thaliana Columbia-0
-
spermidine + N-acetyl-3-aminopropanal + H2O2
-
?
1.5.3.17 spermidine + O2 + H2O Arabidopsis thaliana
-
putrescine + 3-aminobutanal + H2O2
-
?
1.5.3.17 spermidine + O2 + H2O Arabidopsis thaliana Columbia-0
-
putrescine + 3-aminobutanal + H2O2
-
?
1.5.3.17 spermine + O2 + H2O Arabidopsis thaliana
-
spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.17 spermine + O2 + H2O Arabidopsis thaliana Columbia-0
-
spermidine + 3-aminopropanal + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.14 Zea mays
-
-
-
1.5.3.16 Arabidopsis thaliana
-
-
-
1.5.3.16 Arabidopsis thaliana Columbia-0
-
-
-
1.5.3.17 Arabidopsis thaliana
-
-
-
1.5.3.17 Arabidopsis thaliana Q9LYT1 gene AtPAO3
-
1.5.3.17 Arabidopsis thaliana Q9SKX5
-
-
1.5.3.17 Arabidopsis thaliana Columbia-0
-
-
-
1.5.3.17 Arabidopsis thaliana Columbia-0 Q9LYT1 gene AtPAO3
-
1.5.3.17 Arabidopsis thaliana Columbia-0 Q9SKX5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.14 recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography Zea mays
1.5.3.17 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana
1.5.3.17 recombinant maltose-binding protein fusion enzyme from Escherichia coli by amylose affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.17 seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.14 additional information the aminoaldehydes 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal are produced during ZmPAO reaction with spermidine and spermine, respectively Zea mays ?
-
?
1.5.3.14 spermidine + O2 + H2O
-
Zea mays propane-1,3-diamine + 4-aminobutanal + H2O2
-
?
1.5.3.14 spermine + O2 + H2O
-
Zea mays propane-1,3-diamine + N-(3-aminopropyl)-4-aminobutanal + H2O2
-
?
1.5.3.16 additional information no oxidation activity with spermidine Arabidopsis thaliana ?
-
?
1.5.3.16 N1-acetylspermidine + O2 + H2O
-
Arabidopsis thaliana putrescine + 3-acetamidopropanal + H2O2
-
?
1.5.3.16 N1-acetylspermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-acetamidopropanal + H2O2
-
?
1.5.3.16 norspermine + O2 + H2O
-
Arabidopsis thaliana ?
-
?
1.5.3.16 spermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.16 thermospermine + O2 + H2O
-
Arabidopsis thaliana ?
-
?
1.5.3.17 N1-acetylspermidine + O2 + H2O
-
Arabidopsis thaliana putrescine + 3-acetamidopropanal + H2O2
-
?
1.5.3.17 N1-acetylspermidine + O2 + H2O
-
Arabidopsis thaliana Columbia-0 putrescine + 3-acetamidopropanal + H2O2
-
?
1.5.3.17 N1-acetylspermine + O2 + H2O
-
Arabidopsis thaliana spermidine + N-acetyl-3-aminopropanal + H2O2
-
?
1.5.3.17 N1-acetylspermine + O2 + H2O
-
Arabidopsis thaliana Columbia-0 spermidine + N-acetyl-3-aminopropanal + H2O2
-
?
1.5.3.17 norspermine + O2 + H2O
-
Arabidopsis thaliana ?
-
?
1.5.3.17 norspermine + O2 + H2O
-
Arabidopsis thaliana Columbia-0 ?
-
?
1.5.3.17 spermidine + O2 + H2O
-
Arabidopsis thaliana putrescine + 3-aminobutanal + H2O2
-
?
1.5.3.17 spermidine + O2 + H2O
-
Arabidopsis thaliana Columbia-0 putrescine + 3-aminobutanal + H2O2
-
?
1.5.3.17 spermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.17 spermine + O2 + H2O
-
Arabidopsis thaliana Columbia-0 spermidine + 3-aminopropanal + H2O2
-
?
1.5.3.17 thermospermine + O2 + H2O
-
Arabidopsis thaliana ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.14 ZmPAO
-
Zea mays
1.5.3.16 AtPAO1
-
Arabidopsis thaliana
1.5.3.17 AtPAO2
-
Arabidopsis thaliana
1.5.3.17 AtPAO3
-
Arabidopsis thaliana
1.5.3.17 AtPAO4
-
Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.14 25
-
assay at Zea mays
1.5.3.16 25
-
assay at Arabidopsis thaliana
1.5.3.17 25
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.14 32.9
-
spermine pH 7.5, 25°C, recombinant ZmPAO Zea mays
1.5.3.14 50.2
-
spermidine pH 7.5, 25°C, recombinant AtPAO2 Zea mays
1.5.3.16 0.02
-
thermospermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.16 0.09
-
norspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.16 0.2
-
N1-acetylspermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.16 2.5
-
spermine pH 8.0, 25°C, recombinant AtPAO1 Arabidopsis thaliana
1.5.3.17 0.014
-
N1-acetylspermine pH 7.5, 25°C, recombinant AtPAO4 Arabidopsis thaliana
1.5.3.17 0.02
-
N1-acetylspermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.1
-
spermidine pH 7.5, 25°C, recombinant AtPAO4 Arabidopsis thaliana
1.5.3.17 0.1
-
thermospermine pH 7.5, 25°C, recombinant AtPAO4 Arabidopsis thaliana
1.5.3.17 0.4
-
thermospermine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 0.45
-
norspermine pH 7.5, 25°C, recombinant AtPAO4 Arabidopsis thaliana
1.5.3.17 0.5
-
thermospermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 0.8
-
N1-acetylspermine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 1.1
-
norspermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 1.7
-
spermine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 2.9
-
norspermine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 3.4
-
spermidine pH 7.5, 25°C, recombinant AtPAO3 Arabidopsis thaliana
1.5.3.17 4.2
-
spermine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 4.6
-
spermidine pH 7.5, 25°C, recombinant AtPAO2 Arabidopsis thaliana
1.5.3.17 4.6
-
spermine pH 7.5, 25°C, recombinant AtPAO4 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.14 7.5
-
assay at Zea mays
1.5.3.16 8
-
-
Arabidopsis thaliana
1.5.3.17 7 8 dependent on the substrate Arabidopsis thaliana
1.5.3.17 7.5
-
-
Arabidopsis thaliana
1.5.3.17 7.5
-
assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.14 FAD dependent on Zea mays
1.5.3.16 FAD dependent on Arabidopsis thaliana
1.5.3.17 FAD dependent on Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.5.3.14 physiological function plant PAOs oxidize the carbon at the endo-side of the N4-nitrogen of Spd and Spm, producing 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal, respectively, in addition to 1,3-diaminopropane and H2O2, and are considered to be involved in terminal catabolism of polyamines, physiological role(s) of the polyamine catabolic pathways in plants, overview Zea mays
1.5.3.16 evolution AtPAO2-AtPAO4 form a subfamily of polyamine oxidases different from AtPAO1, overview Arabidopsis thaliana
1.5.3.17 evolution AtPAO2-AtPAO4 form a subfamily of polyamine oxxidases different from AtPAO1, EC 1.5.3.16, overview Arabidopsis thaliana
1.5.3.17 metabolism the enzyme is involved in the polyamine back-conversion pathway, overview Arabidopsis thaliana
1.5.3.17 physiological function plant PAOs oxidize the carbon at the endo-side of the N4-nitrogen of Spd and Spm, producing 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal, respectively, in addition to 1,3-diaminopropane and H2O2, and are considered to be involved in terminal catabolism of polyamines, physiological role(s) of the polyamine catabolic pathways in plants, overview. All AtPAOs characterized are involved in a polyamine backconversion pathway Arabidopsis thaliana