Any feedback?
Literature summary extracted from
- Identification of camphor oxidation and reduction products in Pseudomonas putida: new activity of the cytochrome P450cam system (2011), J. Chem. Ecol., 37, 657-667.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.15.1 | the enzyme is CAM plasmid encoded | Pseudomonas putida |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.1 | Fe2+ | the enzyme contains [2Fe2 S] cluster and cytochrome P450 | Pseudomonas putida |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida | under low oxygen conditions borneol is formed instead of 5-ketocamphor | borneol + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida ATCC 17453 | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida ATCC 17453 | under low oxygen conditions borneol is formed instead of 5-ketocamphor | borneol + oxidized putidaredoxin + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.1 | Pseudomonas putida | - |
- |
- |
| 1.14.15.1 | Pseudomonas putida ATCC 17453 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.1 | native C334A enzyme mutant from Pseudomonas putida strain ATCC 17453 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida ATCC 17453 | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions borneol is formed instead of 5-ketocamphor | Pseudomonas putida | borneol + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | the hydroxylation reaction proceeds via a catalytic cycle in which the reduction of dioxygen is coupled to the oxidation of the substrate. A key intermediate in the catalytic cycle is the iron-oxo species (Fe(IV)=O) | Pseudomonas putida | borneol + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions | Pseudomonas putida | borneol + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions borneol is formed instead of 5-ketocamphor | Pseudomonas putida ATCC 17453 | borneol + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | the hydroxylation reaction proceeds via a catalytic cycle in which the reduction of dioxygen is coupled to the oxidation of the substrate. A key intermediate in the catalytic cycle is the iron-oxo species (Fe(IV)=O) | Pseudomonas putida ATCC 17453 | borneol + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions | Pseudomonas putida ATCC 17453 | borneol + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | (+)-exo-5-hydroxycamphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida | 5-oxocamphor + oxidized putidaredoxin + H2O | - |
? | |
| 1.14.15.1 | additional information | under conditions of low oxygen, Pseudomonas putida cells and the isolated P450cam reduce camphor to borneol, product analysis by GC-MS | Pseudomonas putida | ? | - |
? | |
| 1.14.15.1 | additional information | under conditions of low oxygen, Pseudomonas putida cells and the isolated P450cam reduce camphor to borneol, product analysis by GC-MS | Pseudomonas putida ATCC 17453 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.1 | CYP101 | - |
Pseudomonas putida |
| 1.14.15.1 | cytochrome p450cam | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.1 | 22 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.1 | 7.4 | - |
assay at | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.1 | heme | CYP101 is a heme monoxygenase | Pseudomonas putida | |
| 1.14.15.1 | putidaredoxin | - |
Pseudomonas putida |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.15.1 | additional information | P450cam from Pseudomonas putida oxidizes (1R)-(+)-camphor to 5-exo-hydroxy camphor and further to 5-oxo-camphor | Pseudomonas putida |
| 1.14.15.1 | physiological function | Pseudomonas putida is capable of detoxification of camphor and borneol, overview | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
