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Literature summary extracted from

  • Korovashkina, A.; Rymko, A.; Kvach, S.; Zinchenko, A.
    Enzymatic synthesis of c-di-GMP using inclusion bodies of Thermotoga maritima full-length diguanylate cyclase (2013), J. Biotechnol., 164, 276-280.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.65 recombinantly expressed in Escherichia coli. Target protein is produced mainly in the form of inclusion bodies. Both native and mutant enzymes are not inhibited by the target product during conversion of 1 mM GTP into c-di-GMP Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.65 R158A mutant shows increased enzymatic activity compared to wild-type, mutant shows 4fold lower expression level compared to wild-type which might be due to higher enzymatic activity of mutant R158A and its increased toxicity for cells Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.65 Mg2+ Mg2+ required, optimal activity at 2.5 mM MgCl2 Thermotoga maritima
2.7.7.65 NaCl optimal activity at 300-500 mM NaCl Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.65 Thermotoga maritima
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.65 151
-
 pH 10, 60°C, mutant R158A Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.65 GTP
-
 Thermotoga maritima cyclic di-3',5'-guanylate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.65 DGC
-
 Thermotoga maritima
2.7.7.65 diguanylate cyclase
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.65 60
-
-
 Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.65 10
-
-
 Thermotoga maritima