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Literature summary extracted from

  • Yadav, P.K.; Yamada, K.; Chiku, T.; Koutmos, M.; Banerjee, R.
    Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase (2013), J. Biol. Chem., 288, 20002-20013.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.2 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.2 sitting drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5 and 0.02 M betaine hydrochloride Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.2 0.0025
-
 thioredoxin in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.02
-
 3-Mercaptopyruvate glutathione as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.022
-
 3-Mercaptopyruvate using L-cysteine as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.025
-
 3-Mercaptopyruvate using dihydrolipioic acid as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.026
-
 3-Mercaptopyruvate using dithiothreitol as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.03
-
 3-Mercaptopyruvate using L-homocysteine as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.13
-
 3-Mercaptopyruvate using 2-mercaptoethanol as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.35
-
 3-Mercaptopyruvate using cyanide as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.35
-
 3-Mercaptopyruvate using thioredoxin as cosubstrate, in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 4.1
-
 L-cysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 4.4
-
 dihydrolipoic acid in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 4.6
-
 dithiothreitol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 6
-
 cyanide in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 12.5
-
 L-homocysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 28
-
 glutathione in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 108
-
 2-mercaptoethanol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.2 30000
-
 x * 30000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide Homo sapiens
-
 pyruvate + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Homo sapiens P25325
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.2 Ni-NTA agarose column chromatography and DEAE-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + 2-mercaptoethanol
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Homo sapiens pyruvate + thiocyanate
-
 ?
2.8.1.2 3-mercaptopyruvate + dihydrolipoic acid
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + dithiothreitol
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + glutathione
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + L-cysteine
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + L-homocysteine
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + thioredoxin thioredoxin is the preferred persulfide acceptor Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.2 ? x * 30000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.8.1.2 mercaptopyruvate sulfurtransferase
-
 Homo sapiens
2.8.1.2 MST
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.2 0.3
-
 glutathione in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.8
-
 L-homocysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 1.1
-
 L-cysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 1.3
-
 thioredoxin in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 1.7
-
 dihydrolipoic acid in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 2.4
-
 cyanide in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 6.1
-
 dithiothreitol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 229
-
 2-mercaptoethanol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.2 8.2
-
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.1.2 0.01
-
 glutathione in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.06
-
 L-homocysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.27
-
 L-cysteine in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.39
-
 dihydrolipoic acid in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 0.4
-
 cyanide in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 1.3
-
 dithiothreitol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 2.1
-
 2-mercaptoethanol in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens
2.8.1.2 520
-
 thioredoxin in 200 mM of HEPES, pH 7.4, at 37°C Homo sapiens