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Literature summary extracted from

  • Klimacek, M.; Brunsteiner, M.; Nidetzky, B.
    Dynamic mechanism of proton transfer in mannitol 2-dehydrogenase from Pseudomonas fluorescens: mobile GLU292 controls proton relay through a water channel that connects the active site with bulk solvent (2012), J. Biol. Chem., 287, 6655-6667.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.67 E292A mutation partially disrupts the catalytic cycle. Role for residue Glu292 as a gate in a water chain mechanism of proton translocation. Removal of gatekeeper control in the E292A mutant results in a selective, up to 120fold slowing down of microscopicsteps immediately preceding catalytic oxidation of mannitol, consistent with the notion that formation of the productive enzyme-NAD-mannitol complex is promoted by a corresponding position change of Glu292 Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Pseudomonas fluorescens O08355
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-fructose + NADH + H+
-
 Pseudomonas fluorescens D-mannitol + NAD+
-
 r
1.1.1.67 D-mannitol + NAD+
-
 Pseudomonas fluorescens D-fructose + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.67 MtlD
-
 Pseudomonas fluorescens