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Literature summary extracted from
- The deactive form of respiratory complex I from mammalian mitochondria is a Na+/H+ antiporter (2012), J. Biol. Chem., 287, 34743-34751.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | 2-decyl-4-quinazolinyl amine | the compound is both a complex I inhibitor and an uncoupler | Bos taurus |  |
| 7.1.1.2 | fenpyroximate | deactive but not active complex I is a Na+/H+ antiporter that can be inhibited by fenpyroximate | Bos taurus | |
| 7.1.1.2 | additional information | capsaicin, the DELTAlac-acetogenin, 1-methyl-4-phenyl-pyridinium, piericidin A, ranolazine, and stigmatellin have no effect on the antiporter activity, whereas fenpyroximate (as well as rotenone) inhibited it | Bos taurus | |
| 7.1.1.2 | rotenone | deactive but not active complex I is a Na+/H+ antiporter that can be inhibited by rotenone | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.1.2 | mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
| 7.1.1.2 | mitochondrial inner membrane | - |
Yarrowia lipolytica | 5743 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | additional information | Yarrowia lipolytica | no redox-coupled Na+ transport and no Na+/H+ antiporter function by the enzyme from Yarrowia lipolytica | ? | - |
? | |
| 7.1.1.2 | additional information | Bos taurus | no redox-coupled Na+ transport, but the deactive form of complex I, which is formed spontaneously when enzyme turnover is precluded by lack of substrates, is a Na+/H+ antiporter. The antiporter activity is Na+-specific and is abolished upon reactivation by the addition of substrates and by the complex I inhibitor rotenone. It is specific for Na+ over K+ | ? | - |
? | |
| 7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | Bos taurus | - |
NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
| 7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | Yarrowia lipolytica | - |
NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.2 | Bos taurus | - |
- |
- |
| 7.1.1.2 | Yarrowia lipolytica | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.1.1.2 | heart | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | additional information | no redox-coupled Na+ transport and no Na+/H+ antiporter function by the enzyme from Yarrowia lipolytica | Yarrowia lipolytica | ? | - |
? | |
| 7.1.1.2 | additional information | no redox-coupled Na+ transport, but the deactive form of complex I, which is formed spontaneously when enzyme turnover is precluded by lack of substrates, is a Na+/H+ antiporter. The antiporter activity is Na+-specific and is abolished upon reactivation by the addition of substrates and by the complex I inhibitor rotenone. It is specific for Na+ over K+ | Bos taurus | ? | - |
? | |
| 7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | - |
Bos taurus | NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
| 7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | - |
Yarrowia lipolytica | NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | complex I | - |
Bos taurus |
| 7.1.1.2 | complex I | - |
Yarrowia lipolytica |
| 7.1.1.2 | NADH:ubiquinone oxidoreductase | - |
Bos taurus |
| 7.1.1.2 | NADH:ubiquinone oxidoreductase | - |
Yarrowia lipolytica |
| 7.1.1.2 | respiratory complex I | - |
Bos taurus |
| 7.1.1.2 | respiratory complex I | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 32 | 37 | assay at | Bos taurus |
| 7.1.1.2 | 32 | 37 | assay at | Yarrowia lipolytica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 7.5 | - |
assay at | Bos taurus |
| 7.1.1.2 | 7.5 | - |
assay at | Yarrowia lipolytica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | NADH | - |
Bos taurus | |
| 7.1.1.2 | NADH | - |
Yarrowia lipolytica | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | additional information | the deactive state of complex I is formed during hypoxia, when respiratory chain turnover is slowed, and may contribute to determining the outcome of ischemia-reperfusion injury | Bos taurus |
| 7.1.1.2 | physiological function | in mitochondria, complex I (NADH:ubiquinone oxidoreductase) uses the redox potential energy from NADH oxidation by ubiquinone to transport protons across the inner membrane, contributing to the proton-motive force | Bos taurus |
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Release 2023.1 (January 2023)
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