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Literature summary extracted from

  • Bonilla, M.; Denicola, A.; Marino, S.M.; Gladyshev, V.N.; Salinas, G.
    Linked thioredoxin-glutathione systems in platyhelminth parasites: alternative pathways for glutathione reduction and deglutathionylation (2011), J. Biol. Chem., 286, 4959-4967.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.B1 expressed in Escherichia coli BL21(DE3) cells Echinococcus granulosus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.B1 C31S the mutant has negligible deglutathionylase and GR activities when compared with wild type enzyme Echinococcus granulosus
1.8.1.B1 C34S both the deglutathionylase and GR activities of the mutant are only slightly lower when compared with wild type enzyme Echinococcus granulosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.B1 NOC-7 i.e. 1-hydroxy-2-oxo-3-(N-3-methyl-aminopropyl)-3-methyl-1-triazene, complete inhibition at 0.1 mM Echinococcus granulosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.B1 GSSG + NADPH + H+ Echinococcus granulosus
-
 GSH + NADP+
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH + H+ Echinococcus granulosus
-
 thioredoxin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Echinococcus granulosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.B1 Ni-NTA column chromatography Echinococcus granulosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Echinococcus granulosus 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.B1 GSSG + NADPH + H+
-
 Echinococcus granulosus GSH + NADP+
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH + H+
-
 Echinococcus granulosus thioredoxin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.B1 TGR
-
 Echinococcus granulosus
1.8.1.B1 thioredoxin-glutathione reductase
-
 Echinococcus granulosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.B1 FAD
-
 Echinococcus granulosus
1.8.1.B1 NADPH
-
 Echinococcus granulosus