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Literature summary extracted from

  • Parkkinen, T.; Boer, H.; Jaenis, J.; Andberg, M.; Penttilae, M.; Koivula, A.; Rouvinen, J.
    Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens (2011), J. Biol. Chem., 286, 27294-27300.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.203
-
 Agrobacterium tumefaciens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.203 crystal structures of an apo-form, a ternary form in complex with NADH and product, and an inactive Y136A mutant in complex with NAD+. Enzyme is a homohexamer. The monomer contains a Rossmann fold, essential for nucleotide binding. The ternary complex structure reveals a product, D-galactaro-1,5-lactone, which is bound above the nicotinamide ring. This product rearranges in solution to D-galactaro-1,4-lactone Agrobacterium tumefaciens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.203 Y136A inactive. Crystal structure shows changes in the position of residues Ile74 and Ser75. This probably alters the binding of the nicotinamide end of NAD+ Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.203 D-galacturonate + NAD+ + H2O Agrobacterium tumefaciens
-
 D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-galacturonate + NAD+ + H2O Agrobacterium tumefaciens C58 / ATCC 33970
-
 D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O Agrobacterium tumefaciens
-
 D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O Agrobacterium tumefaciens C58 / ATCC 33970
-
 D-glucaric acid + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.203 Agrobacterium tumefaciens Q7CRQ0
-
-
1.1.1.203 Agrobacterium tumefaciens C58 / ATCC 33970 Q7CRQ0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Agrobacterium tumefaciens D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Agrobacterium tumefaciens D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-glucaric acid + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.203 Udh
-
 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.203 NAD+
-
 Agrobacterium tumefaciens