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Literature summary extracted from

  • Yoshida, K., Yamaguchi, M.; Morinaga, T.; Kinehara, M.; Ikeuchi, M.; Ashida, H.; Fujita, Y.
    myo-Inositol catabolism in Bacillus subtilis (2008), J. Biol. Chem., 283, 10415-10424.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.22 expression in Escherichia coli Bacillus subtilis
5.3.1.30 expression in Escherichia coli as a C-terminal His6 tag fusion protein Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.22 3D-3,5/4-trihydroxycyclohexa-1,2-dione + H2O Bacillus subtilis the enzyme is part of the myo-inositol degradation pathway leading to acetyl-CoA 5-deoxy-D-glucuronate
-
 ?
3.7.1.22 3D-3,5/4-trihydroxycyclohexa-1,2-dione + H2O Bacillus subtilis 168 the enzyme is part of the myo-inositol degradation pathway leading to acetyl-CoA 5-deoxy-D-glucuronate
-
 ?
5.3.1.30 5-deoxy-D-glucuronate Bacillus subtilis the enzyme is part of a myo-inositol degradation pathway leading to acetyl-CoA 5-dehydro-2-deoxy-D-gluconate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.22 Bacillus subtilis P42415
-
-
3.7.1.22 Bacillus subtilis 168 P42415
-
-
5.3.1.30 Bacillus subtilis P42413
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.22
-
 Bacillus subtilis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
5.3.1.30 recombinant enzyme Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.22 253
-
 protein extract of Escherichia coli cells that recombinantly express the enzyme, pH and temperature not specified in the publication Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.22 3D-3,5/4-trihydroxycyclohexa-1,2-dione + H2O
-
 Bacillus subtilis 5-deoxy-D-glucuronate
-
 ?
3.7.1.22 3D-3,5/4-trihydroxycyclohexa-1,2-dione + H2O the enzyme is part of the myo-inositol degradation pathway leading to acetyl-CoA Bacillus subtilis 5-deoxy-D-glucuronate
-
 ?
3.7.1.22 3D-3,5/4-trihydroxycyclohexa-1,2-dione + H2O
-
 Bacillus subtilis 168 5-deoxy-D-glucuronate
-
 ?
3.7.1.22 3D-3,5/4-trihydroxycyclohexa-1,2-dione + H2O the enzyme is part of the myo-inositol degradation pathway leading to acetyl-CoA Bacillus subtilis 168 5-deoxy-D-glucuronate
-
 ?
5.3.1.30 5-deoxy-D-glucuronate the enzyme is part of a myo-inositol degradation pathway leading to acetyl-CoA Bacillus subtilis 5-dehydro-2-deoxy-D-gluconate
-
 r
5.3.1.30 5-deoxy-D-glucuronate 5-deoxy-D-glucuronate i.e. 5-deoxy-D-xylo-hexuronic acid Bacillus subtilis 5-dehydro-2-deoxy-D-gluconate 5-dehydro-2-deoxy-D-gluconate i.e. 2-deoxy-D-threo-hex-5-ulosonic acid r

Synonyms

EC Number Synonyms Comment Organism
3.7.1.22 IolD
-
 Bacillus subtilis
3.7.1.22 THcHDO hydrolase
-
 Bacillus subtilis
5.3.1.30 5DG isomerase
-
 Bacillus subtilis
5.3.1.30 IolB
-
 Bacillus subtilis

General Information

EC Number General Information Comment Organism
3.7.1.22 physiological function the enzyme is part of the myo-inositol degradation pathway leading to acetyl-CoA Bacillus subtilis
5.3.1.30 physiological function the enzyme is part of a myo-inositol degradation pathway leading to acetyl-CoA Bacillus subtilis