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Literature summary extracted from

  • Ferri-Fioni, M.L.; Fromant, M.; Bouin, A.P.; Aubard, C.; Lazennec, C.; Plateau, P.; Blanquet, S.
    Identification in archaea of a novel D-Tyr-tRNATyr deacylase (2006), J. Biol. Chem., 281, 27575-27585.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.96 spermidine 0.8 mM, markedly stimulates hydrolysis of D-Tyr-tRNATyr Pyrococcus abyssi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.96 expression in Escherichia coli Archaeoglobus fulgidus
3.1.1.96 expression in Escherichia coli, the archaeal protein forms inclusion bodies Saccharolobus solfataricus
3.1.1.96 overexpression in Escherichia coli Pyrococcus abyssi

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.96 E137A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 the enzyme displays an activity 10fold lower than that of the wild type. The mutant enzyme contains onyl one Zn2+ Archaeoglobus fulgidus
3.1.1.96 E154A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity. The mutant enzyme contains onyl one Zn2+ Archaeoglobus fulgidus
3.1.1.96 H140A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 activity of the H140A variant is so small that it can not be detected, even with 70 nM enzyme in the assay Archaeoglobus fulgidus
3.1.1.96 H141A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 variant exhibits the same activity as that of the wild-type protein Archaeoglobus fulgidus
3.1.1.96 H197A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation increases activity Archaeoglobus fulgidus
3.1.1.96 H80A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity. The mutant enzyme contains onyl one Zn2+ Archaeoglobus fulgidus
3.1.1.96 H92A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity Archaeoglobus fulgidus
3.1.1.96 S78A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation reduces activity Archaeoglobus fulgidus
3.1.1.96 W162A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 mutation increases activity Archaeoglobus fulgidus
3.1.1.96 Y198A in presence of 0.01 mM Zn2+ and 4 mM MgCl2 variant exhibits the same activity as that of the wild-type protein Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.96 EDTA
-
 Archaeoglobus fulgidus
3.1.1.96 EDTA
-
 Pyrococcus abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.96 KCl 200 mM, markedly stimulates hydrolysis of D-Tyr-tRNATyr Pyrococcus abyssi
3.1.1.96 Mg2+ markedly improves initial rate of D-Tyr-tRNATyr hydrolysis. Optimal concentration: 16 mM Pyrococcus abyssi
3.1.1.96 Zn2+ contains strongly bound Zn2+ Pyrococcus abyssi
3.1.1.96 Zn2+ wild-type the enzyme contains two firmly bound Zn2+ Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.96 26959
-
 x * 26959, calculated from sequence Saccharolobus solfataricus
3.1.1.96 30000
-
 x * 30000, SDS-PAGE Saccharolobus solfataricus
3.1.1.96 30781
-
 1 * 30781, calculated from sequence Pyrococcus abyssi
3.1.1.96 30907
-
 1 * 30907, mass spectrometry, SDS-PAGE Pyrococcus abyssi
3.1.1.96 35000
-
 gel filtration Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.96 D-tyrosyl-tRNATyr + H2O Saccharolobus solfataricus the enzyme can recycle misaminoacylated D-Tyr-tRNATyr D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O Pyrococcus abyssi the enzyme can recycle misaminoacylated D-Tyr-tRNATyr D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O Archaeoglobus fulgidus the enzyme can recycle misaminoacylated D-Tyr-tRNATyr D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O Saccharolobus solfataricus P2 the enzyme can recycle misaminoacylated D-Tyr-tRNATyr D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 the enzyme can recycle misaminoacylated D-Tyr-tRNATyr D-tyrosine + tRNATyr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.96 Archaeoglobus fulgidus O29630
-
-
3.1.1.96 Pyrococcus abyssi Q9V2R8
-
-
3.1.1.96 Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 Q9V2R8
-
-
3.1.1.96 Saccharolobus solfataricus Q97WI2
-
-
3.1.1.96 Saccharolobus solfataricus P2 Q97WI2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.96
-
 Saccharolobus solfataricus
3.1.1.96
-
 Pyrococcus abyssi

Storage Stability

EC Number Storage Stability Organism
3.1.1.96 -20°C, 20 mM Tris-HCl buffer, pH 7.8, containing 60% glycerol and 0.05 mM zinc acetate Saccharolobus solfataricus
3.1.1.96 -20°C, 20 mM Tris-HCl buffer, pH 7.8, containing 60% glycerol and 0.05 mM zinc acetate Pyrococcus abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.96 D-tyrosyl-tRNATyr + H2O
-
 Saccharolobus solfataricus D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O
-
 Archaeoglobus fulgidus D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O the enzyme can recycle misaminoacylated D-Tyr-tRNATyr Saccharolobus solfataricus D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O the enzyme can recycle misaminoacylated D-Tyr-tRNATyr Pyrococcus abyssi D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O the enzyme can recycle misaminoacylated D-Tyr-tRNATyr Archaeoglobus fulgidus D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O the initial rate of D-Tyr-tRNATyr deacylation is at least 200fold higher than that of L-Tyr-tRNATyr Pyrococcus abyssi D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O
-
 Saccharolobus solfataricus P2 D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O the enzyme can recycle misaminoacylated D-Tyr-tRNATyr Saccharolobus solfataricus P2 D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O the enzyme can recycle misaminoacylated D-Tyr-tRNATyr Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 D-tyrosine + tRNATyr
-
 ?
3.1.1.96 D-tyrosyl-tRNATyr + H2O the initial rate of D-Tyr-tRNATyr deacylation is at least 200fold higher than that of L-Tyr-tRNATyr Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 D-tyrosine + tRNATyr
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.96 ? x * 30000, SDS-PAGE Saccharolobus solfataricus
3.1.1.96 ? x * 26959, calculated from sequence Saccharolobus solfataricus
3.1.1.96 monomer 1 * 30781, calculated from sequence Pyrococcus abyssi
3.1.1.96 monomer 1 * 30907, mass spectrometry, SDS-PAGE Pyrococcus abyssi

Synonyms

EC Number Synonyms Comment Organism
3.1.1.96 D-Tyr-tRNATyr deacylase
-
 Saccharolobus solfataricus
3.1.1.96 D-Tyr-tRNATyr deacylase
-
 Pyrococcus abyssi
3.1.1.96 D-Tyr-tRNATyr deacylase
-
 Archaeoglobus fulgidus
3.1.1.96 dtd2
-
 Archaeoglobus fulgidus
3.1.1.96 dtd2
-
 Saccharolobus solfataricus
3.1.1.96 dtd2
-
 Pyrococcus abyssi
3.1.1.96 dtdA
-
 Saccharolobus solfataricus
3.1.1.96 dtdA
-
 Pyrococcus abyssi
3.1.1.96 SS02234 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.96 37
-
 assay at Saccharolobus solfataricus
3.1.1.96 37
-
 assay at Pyrococcus abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.96 7.8
-
 assay at Saccharolobus solfataricus
3.1.1.96 7.8
-
 assay at Pyrococcus abyssi