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Literature summary extracted from
- Concerted action of diacetylchitobiose deacetylase and exo-beta-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2004), J. Biol. Chem., 279, 30021-30027.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.33 | expression in Escherichia coli | Thermococcus kodakarensis |
| 3.5.1.105 | expression in Escherichia coli | Thermococcus kodakarensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.33 | 127 | - |
N-acetyl-D-glucosamine | pH 8.5, 75°C | Thermococcus kodakarensis |  |
| 3.5.1.105 | 31.8 | - |
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose | pH 8.5, 75°C | Thermococcus kodakarensis | |
| 3.5.1.105 | 98 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | pH 8.5, 75°C | Thermococcus kodakarensis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.33 | cytoplasm | - |
Thermococcus kodakarensis | 5737 | - |
| 3.5.1.105 | cytoplasm | - |
Thermococcus kodakarensis | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.33 | 30000 | - |
6 * 30000, SDS-PAGE | Thermococcus kodakarensis |
| 3.5.1.33 | 160000 | - |
gel filtration | Thermococcus kodakarensis |
| 3.5.1.105 | 30000 | - |
6 * 30000, SDS-PAGE | Thermococcus kodakarensis |
| 3.5.1.105 | 160000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.33 | N-acetyl-D-glucosamine + H2O | Thermococcus kodakarensis | the enzyme is probably involved in archaeal chitin catabolic pathway | D-glucosamine + acetate | - |
? | |
| 3.5.1.105 | 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O | Thermococcus kodakarensis | i.e. N,N'-diacetylchitobiose, the enzyme is probably involved in archaeal chitin catabolic pathway | 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.33 | Thermococcus kodakarensis | Q6F4N1 | - |
- |
| 3.5.1.105 | Thermococcus kodakarensis | Q6F4N1 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.33 | - |
Thermococcus kodakarensis |
| 3.5.1.105 | - |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.33 | N-acetyl-D-glucosamine + H2O | - |
Thermococcus kodakarensis | D-glucosamine + acetate | - |
? | |
| 3.5.1.33 | N-acetyl-D-glucosamine + H2O | the enzyme is probably involved in archaeal chitin catabolic pathway | Thermococcus kodakarensis | D-glucosamine + acetate | - |
? | |
| 3.5.1.105 | 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O | i.e. N,N'-diacetylchitobiose, the enzyme is probably involved in archaeal chitin catabolic pathway | Thermococcus kodakarensis | 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate | - |
? | |
| 3.5.1.105 | 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H2O | i.e. N,N'-diacetylchitobiose, the deacetylation site is specific to the nonreducing end residue of (GlcNAc)(2). The enzyme can also deacetylate N-acetyl-D-glucosamine (cf. EC 3.5.1.33) | Thermococcus kodakarensis | 2-acetamido-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucose + acetate | - |
? | |
| 3.5.1.105 | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | specific deacetylation at the nonreducing GlcNAc residue | Thermococcus kodakarensis | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcN + acetate | - |
? | |
| 3.5.1.105 | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | specific deacetylation at the nonreducing GlcNAc residue | Thermococcus kodakarensis | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcN + acetate | - |
? | |
| 3.5.1.105 | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O | specific deacetylation at the nonreducing GlcNAc residue | Thermococcus kodakarensis | GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcN + acetate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.33 | homohexamer | 6 * 30000, SDS-PAGE | Thermococcus kodakarensis |
| 3.5.1.105 | homohexamer | 6 * 30000, SDS-PAGE | Thermococcus kodakarensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.33 | Tk-Dac | - |
Thermococcus kodakarensis |
| 3.5.1.33 | TK1764 | locus name | Thermococcus kodakarensis |
| 3.5.1.105 | diacetylchitobiose deacetylase | - |
Thermococcus kodakarensis |
| 3.5.1.105 | GlcNAc2 deacetylase | - |
Thermococcus kodakarensis |
| 3.5.1.105 | Tk-Dac | - |
Thermococcus kodakarensis |
| 3.5.1.105 | TK1764 | locus name | Thermococcus kodakarensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.33 | 70 | - |
assay at | Thermococcus kodakarensis |
| 3.5.1.33 | 75 | - |
- |
Thermococcus kodakarensis |
| 3.5.1.105 | 70 | - |
assay at | Thermococcus kodakarensis |
| 3.5.1.105 | 75 | - |
- |
Thermococcus kodakarensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.105 | 37 | 100 | activity levels at 37°C and 100°C are about 20% of that observed at the optimal temperature (75°C) | Thermococcus kodakarensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.33 | 8 | - |
assay at | Thermococcus kodakarensis |
| 3.5.1.33 | 8.5 | - |
- |
Thermococcus kodakarensis |
| 3.5.1.105 | 8 | - |
assay at | Thermococcus kodakarensis |
| 3.5.1.105 | 8.5 | - |
- |
Thermococcus kodakarensis |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.5.1.33 | Thermococcus kodakarensis | the transcription is specifically induced by N,N'-diacetylchitobiose, suggesting the function of this gene in chitin catabolism in vivo. N-acetyl-D-glucosamine can not act as an inducer | up |
| 3.5.1.105 | Thermococcus kodakarensis | the transcription is specifically induced by GlcNAc2, suggesting the function of this gene in chitin catabolism in vivo. GlcNAc can not act as an inducer | up |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.33 | 0.53 | - |
N-acetyl-D-glucosamine | pH 8.5, 75°C | Thermococcus kodakarensis | |
| 3.5.1.105 | 0.0068 | - |
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc | pH 8.5, 75°C | Thermococcus kodakarensis | |
| 3.5.1.105 | 0.77 | - |
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose | pH 8.5, 75°C | Thermococcus kodakarensis | |
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