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Literature summary extracted from

  • Pierrel, F.; Hernandez, H.L.; Johnson, M.K.; Fontecave, M.; Atta, M.
    MiaB protein from Thermotoga maritima. Characterization of an extremely thermophilic tRNA-methylthiotransferase (2003), J. Biol. Chem., 278, 29515-29524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.4.3 overexpression in Escherichia coli Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.3 [4Fe-4S]-center the sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. Presence of a [4Fe-4S]+2/+1 cluster under reducing and anaerobic conditions, whereas [3Fe-4S]+1 and [2Fe-2S]+2 forms are generated under aerobic conditions Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.3 50000
-
 x * 50000, SDS-PAGE Thermotoga maritima
2.8.4.3 50710
-
 x * 50710, calculated from sequence Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine Thermotoga maritima methylthiolation of N-6-isopentenyl adenosine in tRNAs 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine Thermotoga maritima DSM 3109 methylthiolation of N-6-isopentenyl adenosine in tRNAs 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.3 Thermotoga maritima Q9WZC1
-
-
2.8.4.3 Thermotoga maritima DSM 3109 Q9WZC1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.3
-
 Thermotoga maritima

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-(methylsulfanyl)-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor (overall reaction) Thermotoga maritima
a
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = 2-sulfanyl-N6-dimethylallyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor (1a) Thermotoga maritima
a
2.8.4.3 S-adenosyl-L-methionine + 2-sulfanyl-N6-dimethylallyladenine37 in tRNA = S-adenosyl-L-homocysteine + 2-(methylsulfanyl)-N6-dimethylallyladenine37 in tRNA (1b) Thermotoga maritima
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
-
 Thermotoga maritima 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine methylthiolation of N-6-isopentenyl adenosine in tRNAs Thermotoga maritima 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
-
 Thermotoga maritima DSM 3109 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine methylthiolation of N-6-isopentenyl adenosine in tRNAs Thermotoga maritima DSM 3109 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?

Subunits

EC Number Subunits Comment Organism
2.8.4.3 ? x * 50000, SDS-PAGE Thermotoga maritima
2.8.4.3 ? x * 50710, calculated from sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.8.4.3 2-methylthio-N-6-isopentenyl adenosine synthase
-
 Thermotoga maritima
2.8.4.3 MiaB
-
 Thermotoga maritima
2.8.4.3 TM0653 locus name Thermotoga maritima
2.8.4.3 tRNA-i6A37 methylthiotransferase
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.3 S-adenosyl-L-methionine the enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes Thermotoga maritima

General Information

EC Number General Information Comment Organism
2.8.4.3 physiological function methylthiolation of N-6-isopentenyl adenosine in tRNAs Thermotoga maritima