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Literature summary extracted from

  • Yamazaki, S.; Tsai, L.
    Purification and properties of 8-hydroxy-5-deazaflavin-dependent NADP+ reductase from Methanococcus vannielii (1980), J. Biol. Chem., 255, 6462-6465.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.40 4-Chloromercuriphenyl sulfonate
-
 Methanococcus vannielii
1.5.1.40 iodoacetamide
-
 Methanococcus vannielii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.40 0.0028
-
 1,5-dihydro-8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii
1.5.1.40 0.008
-
 8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii
1.5.1.40 0.0104
-
 NADPH pH 7.0, 20°C Methanococcus vannielii
1.5.1.40 0.0137
-
 NADP+ pH 7.0, 20°C Methanococcus vannielii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.40 43000
-
 2 * 43000, SDS-PAGE Methanococcus vannielii
1.5.1.40 85000
-
 gel filtration Methanococcus vannielii

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.40 Methanococcus vannielii
-
-
-
1.5.1.40 Methanococcus vannielii DSM 1224
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.40
-
 Methanococcus vannielii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.40 1.79
-
 pH 7.0, 20°C Methanococcus vannielii

Storage Stability

EC Number Storage Stability Organism
1.5.1.40 -75°C, 2 months, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), no loss of activity Methanococcus vannielii
1.5.1.40 4°C, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), 22% of the activity is lost Methanococcus vannielii
1.5.1.40 4°C, at low concentration (0.066 mg/ml), the purified enzyme loses 84% of its activity in 2 days Methanococcus vannielii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.40 1,5-dihydro-8-hydroxy-5-deazaflavin + NADP+ the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption Methanococcus vannielii 8-hydroxy-5-deazaflavin + NADPH + H+
-
 r
1.5.1.40 1,5-dihydro-8-hydroxy-5-deazaflavin + NADP+ the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption Methanococcus vannielii DSM 1224 8-hydroxy-5-deazaflavin + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.40 dimer 2 * 43000, SDS-PAGE Methanococcus vannielii

Synonyms

EC Number Synonyms Comment Organism
1.5.1.40 8-hydroxy-5-deazaflavin-dependent NADP+ reductase the enzyme is a component of the formate NADP+ oxidoreductase system of Methanococcus vannielii Methanococcus vannielii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.40 17 20
-
 Methanococcus vannielii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.40 7.37
-
 NADP+ pH 7.0, 20°C Methanococcus vannielii
1.5.1.40 7.37
-
 1,5-dihydro-8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii
1.5.1.40 175
-
 NADPH pH 7.0, 20°C Methanococcus vannielii
1.5.1.40 175
-
 8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.40 4.8
-
 reaction with 8-hydroxy-5-deazaflavin + NADPH Methanococcus vannielii
1.5.1.40 7.9
-
 reaction with 1,5-dihydro-8-hydroxyy-5-deazaflavin + NADP+ Methanococcus vannielii