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Literature summary extracted from

  • Okamoto, N.; Yamaguchi, K.; Mizohata, E.; Tokuoka, K.; Uchiyama, N.; Sugiyama, S.; Matsumura, H.; Inaka, K.; Urade, Y.; Inoue, T.
    Structural insight into the stereoselective production of PGF2alpha by old yellow enzyme from Trypanosoma cruzi (2011), J. Biochem., 150, 563-568.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.99.1 overexpression of the enzyme in Escherichia coli Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.99.1 purified recombinant enzyme in a ternary complex with inhibitor 12-oxophytodienoate and FMN, X-ray diffraction structure determination and analysis at 2.05 A resolution Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.99.1 (9R,13R)-12-oxophytodienoate
-
 Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.99.1 additional information Trypanosoma cruzi old yellow enzyme is an NADPH oxidoreductase capable of reducing a variety of compounds, it catalyzes one- or two-electron reduction ?
-
 ?
1.6.99.1 NADPH + H+ + acceptor Trypanosoma cruzi
-
 NADP+ + reduced acceptor
-
 ?
1.6.99.1 NADPH + H+ + prostaglandin H2 Trypanosoma cruzi via FMN cofactor, stereoselective reaction mechanism NADP+ + prostaglandin F2alpha
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.99.1 Trypanosoma cruzi
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.99.1 NADPH + H+ + acceptor = NADP+ + reduced acceptor reaction mechanism involving a conserved triad of Glu193-His284-Arg249, overview Trypanosoma cruzi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.99.1 additional information old yellow enzyme is an NADPH oxidoreductase capable of reducing a variety of compounds, it catalyzes one- or two-electron reduction Trypanosoma cruzi ?
-
 ?
1.6.99.1 NADPH + H+ + 4-hydroxybenzaldehyde
-
 Trypanosoma cruzi NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + acceptor
-
 Trypanosoma cruzi NADP+ + reduced acceptor
-
 ?
1.6.99.1 NADPH + H+ + prostaglandin H2 via FMN cofactor, stereoselective reaction mechanism Trypanosoma cruzi NADP+ + prostaglandin F2alpha
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.99.1 old yellow enzyme
-
 Trypanosoma cruzi
1.6.99.1 OYE
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.99.1 25
-
 assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.99.1 7
-
 assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.99.1 FMN prosthetic group Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.99.1 0.057
-
 (9R,13R)-12-oxophytodienoate pH 7.0, 25°C Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
1.6.99.1 additional information structure modelling of the enzyme with bound substrate prostaglandin H2 Trypanosoma cruzi