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Literature summary extracted from

  • Chairin, T.; Nitheranont, T.; Watanabe, A.; Asada, Y.; Khanongnuch, C.; Lumyong, S.
    Purification and characterization of the extracellular laccase produced by Trametes polyzona WR710-1 under solid-state fermentation (2013), J. Basic Microbiol., 54, 35-43.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Al3+ 14% inhibition at 5 mM Trametes polyzona
1.10.3.2 Co2+ 28% inhibition at 5 mM Trametes polyzona
1.10.3.2 Cr3+ 23% inhibition at 5 mM Trametes polyzona
1.10.3.2 Cu2+ 19% inhibition at 5 mM Trametes polyzona
1.10.3.2 DTT complete inhibition at 1 mM Trametes polyzona
1.10.3.2 Fe2+ 95% inhibition at 5 mM Trametes polyzona
1.10.3.2 L-cysteine complete inhibition at 10 mM Trametes polyzona
1.10.3.2 Mg2+ 6% inhibition at 5 mM Trametes polyzona
1.10.3.2 NaCl 50% inhibition at 20 mM, the inhibitory effect of chloride ions is reversible Trametes polyzona
1.10.3.2 NaN3 complete inhibition at 0.1 mM Trametes polyzona
1.10.3.2 Zn2+ 19% inhibition at 5 mM Trametes polyzona

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.15
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 0.503
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 1.89
-
 guaiacol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 4.08
-
 catechol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 6.28
-
 L-Dopa pH 5.0, temperature not specified in the publication Trametes polyzona

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Trametes polyzona
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 68000
-
 native enzyme, gel filtration Trametes polyzona
1.10.3.2 71000
-
 1 * 71000, SDS-PAGE Trametes polyzona

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.2 4 catechol + O2 Trametes polyzona
-
 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2 Trametes polyzona WR710-1
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes polyzona
-
-
-
1.10.3.2 Trametes polyzona WR710-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 45fold from solid-state fermentation on peel from Citrus reticulata to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, ultrafiltration, and gel filtration Trametes polyzona

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information laccase from Trametes polyzona WR710-1 is produced under solid-state fermentation using the peel from the Tangerine orange (Citrus reticulata Blanco) as substrate Trametes polyzona
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 60.8
-
 purified native extracellular enzyme, pH 5.0, temperature not specified in the publication, substrate 2,6-dimethoxyphenol Trametes polyzona

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes polyzona ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes polyzona WR710-1 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes polyzona ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes polyzona WR710-1 ?
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes polyzona 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes polyzona WR710-1 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes polyzona ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes polyzona WR710-1 ?
-
 ?
1.10.3.2 L-DOPA + O2
-
 Trametes polyzona ?
-
 ?
1.10.3.2 L-DOPA + O2
-
 Trametes polyzona WR710-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 71000, SDS-PAGE Trametes polyzona

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 purified native enzyme, 60 min, completely stable up to Trametes polyzona
1.10.3.2 50
-
 purified native enzyme, 60 min, 10% loss of activity Trametes polyzona
1.10.3.2 70
-
 purified native enzyme, 60 min, inactivation Trametes polyzona

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 38.2
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 83.2
-
 L-Dopa pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 92.2
-
 guaiacol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 125
-
 catechol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 594
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, temperature not specified in the publication Trametes polyzona

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Trametes polyzona
1.10.3.2 4
-
 with substrates L-DOPA, guaiacol, and catechol Trametes polyzona
1.10.3.2 5
-
 with substrate 2,6-dimethoxyphenol Trametes polyzona

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 6 8 stable within this range Trametes polyzona

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 13.3
-
 L-Dopa pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 30.6
-
 catechol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 48.7
-
 guaiacol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 75.9
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication Trametes polyzona
1.10.3.2 3960
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, temperature not specified in the publication Trametes polyzona