BRENDA - Enzyme Database

PQQ-dependent alcohol dehydrogenase (QEDH) of Pseudomonas aeruginosa is involved in catabolism of acyclic terpenes

Chattopadhyay, A.; Foerster-Fromme, K.; Jendrossek, D.; J. Basic Microbiol. 50, 119-124 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.5.5
gene exaA, expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS RARE
Pseudomonas aeruginosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.5
0.0038
-
ethanol
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
0.0073
-
citronellol
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
0.11
-
geraniol
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
1.3
-
citral
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
2.4
-
citronellal
pH 9.0, 30°C
Pseudomonas aeruginosa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.5
periplasm
-
Pseudomonas aeruginosa
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.5.5
Ca2+
required
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.5.5
ethanol + ubiquinone
Pseudomonas aeruginosa
-
acetaldehyde + ubiquinol
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.5.5
Pseudomonas aeruginosa
-
gene PA1982 or exaA
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.5.5
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS RARE by nickel affinity chromatography
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.5.5
0.1
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-butanediol
Pseudomonas aeruginosa
1.1.5.5
1.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-propandiol
Pseudomonas aeruginosa
1.1.5.5
3.1
-
purified recombinant enzyme, pH 9.0, 30°C, substrate geraniol
Pseudomonas aeruginosa
1.1.5.5
3.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellal
Pseudomonas aeruginosa
1.1.5.5
3.8
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citral
Pseudomonas aeruginosa
1.1.5.5
4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 3-methyl-1--butanol
Pseudomonas aeruginosa
1.1.5.5
4.4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in DMSO; purified recombinant enzyme, pH 9.0, 30°C, substrate 2-propanol
Pseudomonas aeruginosa
1.1.5.5
4.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in DMSO
Pseudomonas aeruginosa
1.1.5.5
5.4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 2-butanol
Pseudomonas aeruginosa
1.1.5.5
5.6
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in H2O
Pseudomonas aeruginosa
1.1.5.5
6.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellol
Pseudomonas aeruginosa
1.1.5.5
7.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-butanol
Pseudomonas aeruginosa
1.1.5.5
7.7
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-propanol
Pseudomonas aeruginosa
1.1.5.5
7.9
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in H2O
Pseudomonas aeruginosa
1.1.5.5
8.2
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-pentanol
Pseudomonas aeruginosa
1.1.5.5
8.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate ethanol
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.5.5
1,3-butandiol + ubiquinone
very low activity, 0.94% of the activity with ethanol
725298
Pseudomonas aeruginosa
? + ubiquinol
-
-
-
?
1.1.5.5
1,3-propandiol + ubiquinone
15% of the activity with ethanol
725298
Pseudomonas aeruginosa
? + ubiquinol
-
-
-
?
1.1.5.5
1-butanol + ubiquinone
88% of the activity with ethanol
725298
Pseudomonas aeruginosa
butanal + ubiquinol
-
-
-
?
1.1.5.5
1-hexanol + ubiquinone
93% of the activity with ethanol
725298
Pseudomonas aeruginosa
hexanal + ubiquinol
-
-
-
?
1.1.5.5
1-octanol + ubiquinone
66% of the activity with ethanol
725298
Pseudomonas aeruginosa
octanal + ubiquinol
-
-
-
?
1.1.5.5
1-pentanol + ubiquinone
97% of the activity with ethanol
725298
Pseudomonas aeruginosa
pentanal + ubiquinol
-
-
-
?
1.1.5.5
1-propanol + ubiquinone
90% of the activity with ethanol
725298
Pseudomonas aeruginosa
propanal + ubiquinol
-
-
-
?
1.1.5.5
2-butanol + ubiquinone
64% of the activity with ethanol
725298
Pseudomonas aeruginosa
2-butanone + ubiquinol
-
-
-
?
1.1.5.5
2-propanol + ubiquinone
51% of the activity with ethanol
725298
Pseudomonas aeruginosa
acetone + ubiquinol
-
-
-
?
1.1.5.5
3-methyl-1-butanol + ubiquinone
48% of the activity with ethanol
725298
Pseudomonas aeruginosa
3-methyl-1-butanal + ubiquinol
-
-
-
?
1.1.5.5
citral + ubiquinol
39% of the activity with ethanol
725298
Pseudomonas aeruginosa
? + ubiquinone
-
-
-
?
1.1.5.5
citronellal + ubiquinol
45% of the activity with ethanol
725298
Pseudomonas aeruginosa
citronellol + ubiquinone
-
-
-
?
1.1.5.5
citronellol + ubiquinone
74% of the activity with ethanol
725298
Pseudomonas aeruginosa
citronellal + ubiquinol
-
-
-
?
1.1.5.5
ethanol + ubiquinone
-
725298
Pseudomonas aeruginosa
acetaldehyde + ubiquinol
-
-
-
?
1.1.5.5
ethanol + ubiquinone
best substrate
725298
Pseudomonas aeruginosa
acetaldehyde + ubiquinol
-
-
-
?
1.1.5.5
geraniol + ubiquinone
37% of the activity with ethanol
725298
Pseudomonas aeruginosa
geranial + ubiquinol
-
-
-
?
1.1.5.5
additional information
substrate specificity, assayed with dichlorophenolindophenol and phenazinemethosulfate as electron acceptors, overview
725298
Pseudomonas aeruginosa
?
-
-
-
-
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.5.5
PQQ-dependent alcohol dehydrogenase
-
Pseudomonas aeruginosa
1.1.5.5
PQQ-dependent ethanol dehydrogenase
-
Pseudomonas aeruginosa
1.1.5.5
QEDH
-
Pseudomonas aeruginosa
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.5.5
30
-
assay at
Pseudomonas aeruginosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.5
9
-
assay at
Pseudomonas aeruginosa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.5
pyrroloquinoline quinone
dependent on, enzyme bound
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.5
gene exaA, expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS RARE
Pseudomonas aeruginosa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.5
pyrroloquinoline quinone
dependent on, enzyme bound
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.5
0.0038
-
ethanol
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
0.0073
-
citronellol
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
0.11
-
geraniol
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
1.3
-
citral
pH 9.0, 30°C
Pseudomonas aeruginosa
1.1.5.5
2.4
-
citronellal
pH 9.0, 30°C
Pseudomonas aeruginosa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.5
periplasm
-
Pseudomonas aeruginosa
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.5.5
Ca2+
required
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.5.5
ethanol + ubiquinone
Pseudomonas aeruginosa
-
acetaldehyde + ubiquinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.5
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS RARE by nickel affinity chromatography
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.5.5
0.1
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-butanediol
Pseudomonas aeruginosa
1.1.5.5
1.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-propandiol
Pseudomonas aeruginosa
1.1.5.5
3.1
-
purified recombinant enzyme, pH 9.0, 30°C, substrate geraniol
Pseudomonas aeruginosa
1.1.5.5
3.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellal
Pseudomonas aeruginosa
1.1.5.5
3.8
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citral
Pseudomonas aeruginosa
1.1.5.5
4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 3-methyl-1--butanol
Pseudomonas aeruginosa
1.1.5.5
4.4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in DMSO; purified recombinant enzyme, pH 9.0, 30°C, substrate 2-propanol
Pseudomonas aeruginosa
1.1.5.5
4.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in DMSO
Pseudomonas aeruginosa
1.1.5.5
5.4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 2-butanol
Pseudomonas aeruginosa
1.1.5.5
5.6
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in H2O
Pseudomonas aeruginosa
1.1.5.5
6.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellol
Pseudomonas aeruginosa
1.1.5.5
7.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-butanol
Pseudomonas aeruginosa
1.1.5.5
7.7
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-propanol
Pseudomonas aeruginosa
1.1.5.5
7.9
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in H2O
Pseudomonas aeruginosa
1.1.5.5
8.2
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-pentanol
Pseudomonas aeruginosa
1.1.5.5
8.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate ethanol
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.5.5
1,3-butandiol + ubiquinone
very low activity, 0.94% of the activity with ethanol
725298
Pseudomonas aeruginosa
? + ubiquinol
-
-
-
?
1.1.5.5
1,3-propandiol + ubiquinone
15% of the activity with ethanol
725298
Pseudomonas aeruginosa
? + ubiquinol
-
-
-
?
1.1.5.5
1-butanol + ubiquinone
88% of the activity with ethanol
725298
Pseudomonas aeruginosa
butanal + ubiquinol
-
-
-
?
1.1.5.5
1-hexanol + ubiquinone
93% of the activity with ethanol
725298
Pseudomonas aeruginosa
hexanal + ubiquinol
-
-
-
?
1.1.5.5
1-octanol + ubiquinone
66% of the activity with ethanol
725298
Pseudomonas aeruginosa
octanal + ubiquinol
-
-
-
?
1.1.5.5
1-pentanol + ubiquinone
97% of the activity with ethanol
725298
Pseudomonas aeruginosa
pentanal + ubiquinol
-
-
-
?
1.1.5.5
1-propanol + ubiquinone
90% of the activity with ethanol
725298
Pseudomonas aeruginosa
propanal + ubiquinol
-
-
-
?
1.1.5.5
2-butanol + ubiquinone
64% of the activity with ethanol
725298
Pseudomonas aeruginosa
2-butanone + ubiquinol
-
-
-
?
1.1.5.5
2-propanol + ubiquinone
51% of the activity with ethanol
725298
Pseudomonas aeruginosa
acetone + ubiquinol
-
-
-
?
1.1.5.5
3-methyl-1-butanol + ubiquinone
48% of the activity with ethanol
725298
Pseudomonas aeruginosa
3-methyl-1-butanal + ubiquinol
-
-
-
?
1.1.5.5
citral + ubiquinol
39% of the activity with ethanol
725298
Pseudomonas aeruginosa
? + ubiquinone
-
-
-
?
1.1.5.5
citronellal + ubiquinol
45% of the activity with ethanol
725298
Pseudomonas aeruginosa
citronellol + ubiquinone
-
-
-
?
1.1.5.5
citronellol + ubiquinone
74% of the activity with ethanol
725298
Pseudomonas aeruginosa
citronellal + ubiquinol
-
-
-
?
1.1.5.5
ethanol + ubiquinone
-
725298
Pseudomonas aeruginosa
acetaldehyde + ubiquinol
-
-
-
?
1.1.5.5
ethanol + ubiquinone
best substrate
725298
Pseudomonas aeruginosa
acetaldehyde + ubiquinol
-
-
-
?
1.1.5.5
geraniol + ubiquinone
37% of the activity with ethanol
725298
Pseudomonas aeruginosa
geranial + ubiquinol
-
-
-
?
1.1.5.5
additional information
substrate specificity, assayed with dichlorophenolindophenol and phenazinemethosulfate as electron acceptors, overview
725298
Pseudomonas aeruginosa
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.5.5
30
-
assay at
Pseudomonas aeruginosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.5
9
-
assay at
Pseudomonas aeruginosa
General Information
EC Number
General Information
Commentary
Organism
1.1.5.5
malfunction
inactivation of PA1982 by insertion mutagenesis results in inability of the mutant to utilise ethanol and in reduced growth on geraniol. Growth on ethanol is restored by transferring an intact copy of the PA1982 gene into the mutant
Pseudomonas aeruginosa
1.1.5.5
physiological function
the PQQ-dependent alcohol dehydrogenase of Pseudomonas aeruginosa functions in ethanol metabolism and is involved in catabolism of acyclic terpenes, overview
Pseudomonas aeruginosa
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.5.5
malfunction
inactivation of PA1982 by insertion mutagenesis results in inability of the mutant to utilise ethanol and in reduced growth on geraniol. Growth on ethanol is restored by transferring an intact copy of the PA1982 gene into the mutant
Pseudomonas aeruginosa
1.1.5.5
physiological function
the PQQ-dependent alcohol dehydrogenase of Pseudomonas aeruginosa functions in ethanol metabolism and is involved in catabolism of acyclic terpenes, overview
Pseudomonas aeruginosa