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Literature summary extracted from
- Identification of 3-sulfinopropionyl coenzyme A (CoA) desulfinases within the acyl-CoA dehydrogenase superfamily (2014), J. Bacteriol., 196, 882-893.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.13.1.4 | heterologously expressed in Escherichia coli BL21 | Cupriavidus necator |
| 3.13.1.4 | heterologously expressed in Escherichia coli BL21 | Variovorax paradoxus |
| 3.13.1.4 | heterologously expressed in Escherichia coli BL21 | Paraburkholderia xenovorans |
| 3.13.1.4 | heterologously expressed in Escherichia coli BL21 | Advenella mimigardefordensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.1.4 | 0.013 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C, with a Vmax of 4.19 micromol/min*mg | Advenella mimigardefordensis |  |
| 3.13.1.4 | 0.027 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C, with a Vmax of 1.58 micromol/min*mg | Paraburkholderia xenovorans | |
| 3.13.1.4 | 0.052 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C, with a Vmax of 3.47 micromol/min*mg | Variovorax paradoxus | |
| 3.13.1.4 | 0.078 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C, with a Vmax of 6.1 micromol/min*mg | Cupriavidus necator | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.4 | 140000 | - |
experimentyl, gel filtration | Paraburkholderia xenovorans |
| 3.13.1.4 | 157000 | - |
experimental, gel filtration | Cupriavidus necator |
| 3.13.1.4 | 157000 | - |
experimental, gel filtration | Variovorax paradoxus |
| 3.13.1.4 | 172000 | - |
theoretical, calculated | Paraburkholderia xenovorans |
| 3.13.1.4 | 173000 | - |
experimental, gel filtarion | Advenella mimigardefordensis |
| 3.13.1.4 | 174000 | - |
theoretical, calculated | Variovorax paradoxus |
| 3.13.1.4 | 175000 | - |
theoretical, calculated | Cupriavidus necator |
| 3.13.1.4 | 175000 | - |
theoretical, calculated | Advenella mimigardefordensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | Cupriavidus necator | - |
propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | Variovorax paradoxus | - |
propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | Paraburkholderia xenovorans | - |
propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | Advenella mimigardefordensis | - |
propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | Advenella mimigardefordensis DPN7 | - |
propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | Cupriavidus necator N-1 | - |
propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | Variovorax paradoxus TBEA6 | - |
propionyl-CoA + sulfite | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.1.4 | Advenella mimigardefordensis | - |
- |
- |
| 3.13.1.4 | Advenella mimigardefordensis DPN7 | - |
- |
- |
| 3.13.1.4 | Cupriavidus necator | - |
- |
- |
| 3.13.1.4 | Cupriavidus necator N-1 | - |
- |
- |
| 3.13.1.4 | Paraburkholderia xenovorans | - |
- |
- |
| 3.13.1.4 | Variovorax paradoxus | - |
- |
- |
| 3.13.1.4 | Variovorax paradoxus TBEA6 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.1.4 | recombinant protein | Cupriavidus necator |
| 3.13.1.4 | recombinant protein | Variovorax paradoxus |
| 3.13.1.4 | recombinant protein | Paraburkholderia xenovorans |
| 3.13.1.4 | recombinant protein | Advenella mimigardefordensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | - |
Cupriavidus necator | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | - |
Variovorax paradoxus | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | - |
Paraburkholderia xenovorans | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | - |
Advenella mimigardefordensis | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | tested in a continuous spectrophotometric assay | Cupriavidus necator | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | tested in a continuous spectrophotometric assay | Variovorax paradoxus | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | tested in a continuous spectrophotometric assay | Paraburkholderia xenovorans | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | tested in a continuous spectrophotometric assay | Advenella mimigardefordensis | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | - |
Advenella mimigardefordensis DPN7 | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | tested in a continuous spectrophotometric assay | Advenella mimigardefordensis DPN7 | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | - |
Cupriavidus necator N-1 | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | tested in a continuous spectrophotometric assay | Cupriavidus necator N-1 | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | - |
Variovorax paradoxus TBEA6 | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | 3-sulfinopropionyl-CoA + H2O | tested in a continuous spectrophotometric assay | Variovorax paradoxus TBEA6 | propionyl-CoA + sulfite | - |
? | |
| 3.13.1.4 | additional information | shows high amino acid sequence similarity of acyl-CoA dehydrogenases, but no dehydrogenation activity of acyl-CoA thioesters (butyryl-CoA, isobutyryl-CoA, and valeryl-CoA) is observed | Cupriavidus necator | ? | - |
? | |
| 3.13.1.4 | additional information | shows high amino acid sequence similarity of acyl-CoA dehydrogenases, but no dehydrogenation activity of acyl-CoA thioesters (butyryl-CoA, isobutyryl-CoA, and valeryl-CoA) is observed | Variovorax paradoxus | ? | - |
? | |
| 3.13.1.4 | additional information | shows high amino acid sequence similarity of acyl-CoA dehydrogenases, but no dehydrogenation activity of acyl-CoA thioesters (butyryl-CoA, isobutyryl-CoA, and valeryl-CoA) is observed | Paraburkholderia xenovorans | ? | - |
? | |
| 3.13.1.4 | additional information | shows high amino acid sequence similarity of acyl-CoA dehydrogenases, but no dehydrogenation activity of acyl-CoA thioesters (butyryl-CoA, isobutyryl-CoA, and valeryl-CoA) is observed | Advenella mimigardefordensis | ? | - |
? | |
| 3.13.1.4 | additional information | shows high amino acid sequence similarity of acyl-CoA dehydrogenases, but no dehydrogenation activity of acyl-CoA thioesters (butyryl-CoA, isobutyryl-CoA, and valeryl-CoA) is observed | Advenella mimigardefordensis DPN7 | ? | - |
? | |
| 3.13.1.4 | additional information | shows high amino acid sequence similarity of acyl-CoA dehydrogenases, but no dehydrogenation activity of acyl-CoA thioesters (butyryl-CoA, isobutyryl-CoA, and valeryl-CoA) is observed | Cupriavidus necator N-1 | ? | - |
? | |
| 3.13.1.4 | additional information | shows high amino acid sequence similarity of acyl-CoA dehydrogenases, but no dehydrogenation activity of acyl-CoA thioesters (butyryl-CoA, isobutyryl-CoA, and valeryl-CoA) is observed | Variovorax paradoxus TBEA6 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.1.4 | homotetramer | - |
Advenella mimigardefordensis |
| 3.13.1.4 | tri- or tetramer | - |
Cupriavidus necator |
| 3.13.1.4 | tri- or tetramer | - |
Variovorax paradoxus |
| 3.13.1.4 | tri- or tetramer | - |
Paraburkholderia xenovorans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.13.1.4 | 3-sulfinopropionyl CoA desulfinase | - |
Cupriavidus necator |
| 3.13.1.4 | 3-sulfinopropionyl CoA desulfinase | - |
Variovorax paradoxus |
| 3.13.1.4 | 3-sulfinopropionyl CoA desulfinase | - |
Paraburkholderia xenovorans |
| 3.13.1.4 | 3-sulfinopropionyl CoA desulfinase | - |
Advenella mimigardefordensis |
| 3.13.1.4 | 3-sulfinopropionyl coenzyme A desulfinase | - |
Cupriavidus necator |
| 3.13.1.4 | 3-sulfinopropionyl coenzyme A desulfinase | - |
Variovorax paradoxus |
| 3.13.1.4 | 3-sulfinopropionyl coenzyme A desulfinase | - |
Paraburkholderia xenovorans |
| 3.13.1.4 | 3-sulfinopropionyl coenzyme A desulfinase | - |
Advenella mimigardefordensis |
| 3.13.1.4 | 3SP-CoA desulfinase | - |
Cupriavidus necator |
| 3.13.1.4 | 3SP-CoA desulfinase | - |
Variovorax paradoxus |
| 3.13.1.4 | 3SP-CoA desulfinase | - |
Paraburkholderia xenovorans |
| 3.13.1.4 | 3SP-CoA desulfinase | - |
Advenella mimigardefordensis |
| 3.13.1.4 | AcdDPN7 | - |
Advenella mimigardefordensis |
| 3.13.1.4 | AcdLB400 | - |
Paraburkholderia xenovorans |
| 3.13.1.4 | AcdN-1 | - |
Cupriavidus necator |
| 3.13.1.4 | AcdTBEA6 | - |
Variovorax paradoxus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.4 | 30 | - |
assay at | Cupriavidus necator |
| 3.13.1.4 | 30 | - |
assay at | Variovorax paradoxus |
| 3.13.1.4 | 30 | - |
assay at | Paraburkholderia xenovorans |
| 3.13.1.4 | 30 | - |
assay at | Advenella mimigardefordensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.1.4 | 1.1 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Paraburkholderia xenovorans | |
| 3.13.1.4 | 2.5 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Variovorax paradoxus | |
| 3.13.1.4 | 3.1 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Advenella mimigardefordensis | |
| 3.13.1.4 | 4.4 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Cupriavidus necator | |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.1.4 | 7.4 | - |
assay at | Cupriavidus necator |
| 3.13.1.4 | 7.4 | - |
assay at | Variovorax paradoxus |
| 3.13.1.4 | 7.4 | - |
assay at | Paraburkholderia xenovorans |
| 3.13.1.4 | 7.4 | - |
assay at | Advenella mimigardefordensis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.1.4 | FAD | tightly bound | Cupriavidus necator | |
| 3.13.1.4 | FAD | tightly bound | Variovorax paradoxus | |
| 3.13.1.4 | FAD | tightly bound | Paraburkholderia xenovorans | |
| 3.13.1.4 | FAD | tightly bound | Advenella mimigardefordensis | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.13.1.4 | Advenella mimigardefordensis | calculated | - |
6.7 |
| 3.13.1.4 | Cupriavidus necator | - |
- |
7.9 |
| 3.13.1.4 | Variovorax paradoxus | - |
- |
8.5 |
| 3.13.1.4 | Paraburkholderia xenovorans | - |
- |
8.8 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.1.4 | 41.7 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Paraburkholderia xenovorans | |
| 3.13.1.4 | 48.5 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Variovorax paradoxus | |
| 3.13.1.4 | 57 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Cupriavidus necator | |
| 3.13.1.4 | 240.8 | - |
3-sulfinopropionyl-CoA | pH 7.4, 37°C | Advenella mimigardefordensis | |
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