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Literature summary extracted from

  • Jung, J.H.; Seo, D.H.; Holden, J.F.; Park, C.S.
    Identification and characterization of an archaeal kojibiose catabolic pathway in the hyperthermophilic Pyrococcus sp. strain ST04 (2014), J. Bacteriol., 196, 1122-1131.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.230 heterologous expression in Escherichia coli Pyrococcus sp.
5.4.2.6 expression in Escherichia coli Pyrococcus sp.
5.4.2.6 heterologous expression in Escherichia coli Pyrococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.230 MnCl2 1 mM, 34% loss of activity Pyrococcus sp.
2.4.1.230 additional information 1 mM EDTA does not effect activity Pyrococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.230 1.34
-
 phosphate pH 6.0, 90°C Pyrococcus sp.
2.4.1.230 2.5 3 2-alpha-D-glucosyl-D-glucose pH 6.0, 90°C Pyrococcus sp.
2.4.1.230 36.8
-
 D-glucose pH 6.0, 90°C Pyrococcus sp.
5.4.2.6 2.08
-
 beta-D-glucose 1-phosphate pH 6.0, 90°C Pyrococcus sp.
5.4.2.6 2.08
-
 beta-D-glucose 1-phosphate 90°C, pH not specified in the publication Pyrococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.230 CdCl2 1 mM, elevates hydrolysis activity by 33% Pyrococcus sp.
2.4.1.230 CuCl2 1 mM, elevates hydrolysis activity by 40% Pyrococcus sp.
2.4.1.230 additional information the enzyme does not require metal ions Pyrococcus sp.
2.4.1.230 ZnCl2 1 mM, elevates hydrolysis activity by 36% Pyrococcus sp.
5.4.2.6 Mg2+ conversion of beta-D-glucose 1-phosphate into beta-D-glucose 6-phosphate in the presence of 6 mM MgCl2 Pyrococcus sp.
5.4.2.6 Mg2+ no activity without Mg2+. 6 mM is the optimal concentration Pyrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.230 80000
-
 x * 80000, SDS-PAGE Pyrococcus sp.
5.4.2.6 25000
-
 x * 25000, SDS-PAGE Pyrococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.230 2-alpha-D-glucosyl-D-glucose + phosphate Pyrococcus sp. 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea D-glucose + beta-D-glucose 1-phosphate
-
 ?
2.4.1.230 2-alpha-D-glucosyl-D-glucose + phosphate Pyrococcus sp. ST04 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea D-glucose + beta-D-glucose 1-phosphate
-
 ?
5.4.2.6 beta-D-Glucose 1-phosphate Pyrococcus sp. the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate Pyrococcus sp. the enzyme is involved in the kojibiose catabolic pathway beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate Pyrococcus sp. ST04 the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate Pyrococcus sp. ST04 the enzyme is involved in the kojibiose catabolic pathway beta-D-Glucose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.230 Pyrococcus sp. I3RFR4
-
-
2.4.1.230 Pyrococcus sp. ST04 I3RFR4
-
-
5.4.2.6 Pyrococcus sp. I3RFR5
-
-
5.4.2.6 Pyrococcus sp. ST04 I3RFR5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.230 recombinant enzyme Pyrococcus sp.
5.4.2.6
-
 Pyrococcus sp.
5.4.2.6 recombinant enzyme Pyrococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.230 10.7
-
 pH 6.0, 80°C, substrate: kojibiose Pyrococcus sp.
5.4.2.6 3.51
-
 90°C, pH not specified in the publication, conversion of beta-D-glucose 6-phosphate into beta-D-glucose 1-phosphate Pyrococcus sp.
5.4.2.6 3.51
-
 pH 6.0, 90°C, conversion from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate Pyrococcus sp.
5.4.2.6 46.81
-
 90°C, pH not specified in the publication, conversion of beta-D-glucose 1-phosphate into beta-D-glucose 6-phosphate Pyrococcus sp.
5.4.2.6 46.81
-
 pH 6.0, 90°C, conversion from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate Pyrococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.230 2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. D-glucose + beta-D-glucose 1-phosphate
-
 ?
2.4.1.230 2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose Pyrococcus sp. D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.230 2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose. The enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. ST04 D-glucose + beta-D-glucose 1-phosphate
-
 ?
2.4.1.230 2-alpha-D-glucosyl-D-glucose + phosphate 2-alpha-D-glucosyl-D-glucose i.e. kojibiose Pyrococcus sp. ST04 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.230 3-O-alpha-D-glucosyl-D-glucose + phosphate 1-alpha-D-glucosyl-D-glucose i.e. nigerose, at 0.23% of the activity with kojibiose Pyrococcus sp. D-glucose + beta-D-glucose 1-phosphate
-
 ?
2.4.1.230 3-O-alpha-D-glucosyl-D-glucose + phosphate 1-alpha-D-glucosyl-D-glucose i.e. nigerose, at 0.23% of the activity with kojibiose Pyrococcus sp. ST04 D-glucose + beta-D-glucose 1-phosphate
-
 ?
2.4.1.230 additional information no activity with trehalose or cellobiose. Activity with maltose or isomaltose es less than 0.001% of the activity with kojibiose Pyrococcus sp. ?
-
 ?
2.4.1.230 additional information no activity with trehalose or cellobiose. Activity with maltose or isomaltose es less than 0.001% of the activity with kojibiose Pyrococcus sp. ST04 ?
-
 ?
5.4.2.6 beta-D-Glucose 1-phosphate
-
 Pyrococcus sp. beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate the enzyme is involved in the kojibiose catabolic pathway Pyrococcus sp. beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate conversion activity from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate is 46.81 U/mg, and reverse conversion activity from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate is 3.51 U/mg Pyrococcus sp. beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate
-
 Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate the enzyme is involved in the kojibiose catabolic pathway Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
 r
5.4.2.6 beta-D-Glucose 1-phosphate conversion activity from beta-D-glucose 1-phosphate to beta-D-glucose 6-phosphate is 46.81 U/mg, and reverse conversion activity from beta-D-glucose 6-phosphate to beta-D-glucose 1-phosphate is 3.51 U/mg Pyrococcus sp. ST04 beta-D-Glucose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.230 ? x * 80000, SDS-PAGE Pyrococcus sp.
5.4.2.6 ? x * 25000, SDS-PAGE Pyrococcus sp.

Synonyms

EC Number Synonyms Comment Organism
2.4.1.230 kojibiose phosphorylase
-
 Pyrococcus sp.
2.4.1.230 PsKP
-
 Pyrococcus sp.
2.4.1.230 Py04_1502 locus name Pyrococcus sp.
5.4.2.6 beta-phosphoglucomutase
-
 Pyrococcus sp.
5.4.2.6 PsPGM
-
 Pyrococcus sp.
5.4.2.6 Py04_1503 locus name Pyrococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.230 90
-
-
 Pyrococcus sp.
5.4.2.6 95
-
-
 Pyrococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.230 70
-
 20 min, stable Pyrococcus sp.
2.4.1.230 95
-
 half-life: 71 h Pyrococcus sp.
2.4.1.230 100
-
 2 h, over 40% of the activity remains. Half-life: 1.9 h Pyrococcus sp.
5.4.2.6 70
-
 20 min, stable Pyrococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.230 37.7
-
 phosphate pH 6.0, 90°C Pyrococcus sp.
2.4.1.230 37.7
-
 2-alpha-D-glucosyl-D-glucose pH 6.0, 90°C Pyrococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.230 5
-
-
 Pyrococcus sp.
2.4.1.230 6
-
 assay at Pyrococcus sp.
5.4.2.6 6
-
-
 Pyrococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.230 4 6 over 90% of maximal activity at pH 4.0 and at pH 6.0 Pyrococcus sp.
5.4.2.6 4 9
-
 Pyrococcus sp.

General Information

EC Number General Information Comment Organism
2.4.1.230 physiological function the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp.
5.4.2.6 physiological function the enzyme is involved in conversion of kojibiose into beta-D-glucose 6-phosphate, a substrate of the glycolytic pathway. Disaccharide utilization pathway via phosphorolysis in hyperthermophilic archaea Pyrococcus sp.
5.4.2.6 physiological function the enzyme is involved in the kojibiose catabolic pathway Pyrococcus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.230 14.9
-
 2-alpha-D-glucosyl-D-glucose pH 6.0, 90°C Pyrococcus sp.
2.4.1.230 28.1
-
 phosphate pH 6.0, 90°C Pyrococcus sp.
5.4.2.6 9.07
-
 beta-D-glucose 1-phosphate 90°C, pH not specified in the publication Pyrococcus sp.
5.4.2.6 9.07
-
 beta-D-glucose 1-phosphate pH 6.0, 90°C Pyrococcus sp.