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Literature summary extracted from

  • Aono, R.; Sato, T.; Yano, A.; Yoshida, S.; Nishitani, Y.; Miki, K.; Imanaka, T.; Atomi, H.
    Enzymatic characterization of AMP phosphorylase and ribose-1,5-bisphosphate isomerase functioning in an archaeal AMP metabolic pathway (2012), J. Bacteriol., 194, 6847-6855.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.57 cAMP the activity with CMP and UMP requires activation by cAMP Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.57 additional information
-
 AMP the kinetic parameters of the enzyme toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
2.4.2.57 2.8
-
 phosphate pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 2.8
-
 phosphate pH 7.5, 85°C, cosubstrate: AMP Thermococcus kodakarensis
2.4.2.57 4.4
-
 UMP pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 6.2
-
 CMP pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 18.5
-
 GMP pH 7.5, 85°C Thermococcus kodakarensis
5.3.1.29 0.6
-
 alpha-D-ribose 1,5-bisphosphate pH 8.3, 85°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.57 AMP + phosphate Thermococcus kodakarensis the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
2.4.2.57 AMP + phosphate Thermococcus kodakarensis the enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
5.3.1.29 alpha-D-ribose 1,5-bisphosphate Thermococcus kodakarensis the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes D-ribulose 1,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.57 Thermococcus kodakarensis Q5JCX3
-
-
5.3.1.29 Thermococcus kodakarensis Q5JFM9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.57 recombinant enzyme Thermococcus kodakarensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.57 AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate (1) Thermococcus kodakarensis
a
2.4.2.57 CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate (2) Thermococcus kodakarensis
a
2.4.2.57 UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate (3) Thermococcus kodakarensis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.57 1.8
-
 substrate: GMP, pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 10.8
-
 substrate: UMP, pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 15.9
-
 substrate: AMP, pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 37.5
-
 substrate: CMP, pH 7.5, 85°C Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.57 AMP + phosphate
-
 Thermococcus kodakarensis adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
2.4.2.57 AMP + phosphate the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes Thermococcus kodakarensis adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
2.4.2.57 AMP + phosphate the enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes Thermococcus kodakarensis adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
2.4.2.57 CMP + phosphate
-
 Thermococcus kodakarensis cytosine + alpha-D-ribose 1,5-bisphosphate
-
 ?
2.4.2.57 GMP + phosphate
-
 Thermococcus kodakarensis guanine + alpha-D-ribose 1,5-bisphosphate
-
 ?
2.4.2.57 additional information the use of dAMP, dGMP, or dUMP results in the generation of only trace amounts of their corresponding nucleobases, adenine, guanine, or uracil, respectivelyno activity with: TMP, ADP, ATP, dCMP, dAMP, dGMP, dUMP Thermococcus kodakarensis ?
-
 ?
2.4.2.57 UMP + phosphate
-
 Thermococcus kodakarensis uracil + alpha-D-ribose 1,5-bisphosphate
-
 ?
5.3.1.29 alpha-D-ribose 1,5-bisphosphate the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes Thermococcus kodakarensis D-ribulose 1,5-bisphosphate
-
 ?
5.3.1.29 alpha-D-ribose 1,5-bisphosphate the enzyme is specific for the alpha-anomer of D-ribose 1,5-bisphosphate and does not recognize other sugar compounds (ribose 5-phosphate, phosphoribosylpyrophosphate, fructose 1,6-bisphosphate, fructose 6-phosphate, glucose, pyruvate or 3-phosphoglycerate) Thermococcus kodakarensis D-ribulose 1,5-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.57 AMPpase
-
 Thermococcus kodakarensis
2.4.2.57 deoA
-
 Thermococcus kodakarensis
2.4.2.57 nucleoside monophosphate phosphorylase
-
 Thermococcus kodakarensis
5.3.1.29 R15P isomerase
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.57 85
-
 assay at Thermococcus kodakarensis
2.4.2.57 85
-
 assay Thermococcus kodakarensis
5.3.1.29 85
-
 assay at Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.57 additional information
-
 additional information the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
2.4.2.57 additional information
-
 AMP the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
2.4.2.57 2.7
-
 GMP pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 10.5
-
 UMP pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 15
-
 phosphate pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 15
-
 phosphate pH 7.5, 85°C, cosubstrate: AMP Thermococcus kodakarensis
2.4.2.57 39.1
-
 CMP pH 7.5, 85°C Thermococcus kodakarensis
5.3.1.29 29.2
-
 alpha-D-ribose 1,5-bisphosphate pH 8.3, 85°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.57 7.5
-
 assay at Thermococcus kodakarensis
5.3.1.29 8.3
-
 assay at Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.1.29 AMP activity is extremely low with the substrate alpha-D-ribose 1,5-bisphosphate alone but is more than 40fold activated in the presence of AMP Thermococcus kodakarensis

Expression

EC Number Organism Comment Expression
5.3.1.29 Thermococcus kodakarensis increased protein levels of the ribose 1,5-bisphosphate isomerase in the cells cultivated with nucleosides up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.57 additional information
-
 additional information the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
2.4.2.57 additional information
-
 AMP the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
2.4.2.57 0.1
-
 GMP pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 2.4
-
 UMP pH 7.5, 85°C Thermococcus kodakarensis
2.4.2.57 5.4
-
 phosphate pH 7.5, 85°C, cosubstrate: AMP Thermococcus kodakarensis
2.4.2.57 6.3
-
 CMP pH 7.5, 85°C Thermococcus kodakarensis
5.3.1.29 48.7
-
 alpha-D-ribose 1,5-bisphosphate pH 8.3, 85°C Thermococcus kodakarensis