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Literature summary extracted from

  • Newell, P.; Yoshioka, S.; Hvorecny, K.; Monds, R.; OToole, G.
    Systematic analysis of diguanylate cyclases that promote biofilm formation by Pseudomonas fluorescens Pf0-1 (2011), J. Bacteriol., 193, 4685-4698.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.65 acetyl phosphate
-
 Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.65 addition of a C-terminal His-tag to GcbC results in a loss of function and stability Pseudomonas fluorescens
2.7.7.65 expressed in Escherichia coli as a His-tagged fusion protein Pseudomonas fluorescens
2.7.7.65 stably expressed in Escherichia coli as a His-tagged fusion protein Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.65 additional information mutant bearing a GGDEF-to-GGAAF mutation is able to complement a deletion mutant Pseudomonas fluorescens
2.7.7.65 additional information mutant bearing a GGDEF-to-GGAAF mutation is not able to complement a deletion mutant Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.65 Pseudomonas fluorescens
-
-
-
2.7.7.65 Pseudomonas fluorescens Q3K751
-
-
2.7.7.65 Pseudomonas fluorescens Q3KFC4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.65 GTP
-
 Pseudomonas fluorescens cyclic di-3',5'-guanylate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.65 diguanylate cyclase
-
 Pseudomonas fluorescens
2.7.7.65 gcbB
-
 Pseudomonas fluorescens
2.7.7.65 gcbC
-
 Pseudomonas fluorescens
2.7.7.65 Pfl01_1058
-
 Pseudomonas fluorescens
2.7.7.65 Pfl01_1789
-
 Pseudomonas fluorescens
2.7.7.65 Pfl01_4666
-
 Pseudomonas fluorescens
2.7.7.65 WspR
-
 Pseudomonas fluorescens

General Information

EC Number General Information Comment Organism
2.7.7.65 malfunction a deletion mutant shows a marked reduction in biofilm formation Pseudomonas fluorescens