EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.74 | cytoplasmic domain of IPCT is expressed in Escherichia coli as a His-tagged fusion protein | Archaeoglobus fulgidus |
2.7.7.74 | the cytoplasmic domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) is produced in Escherichia coli | Archaeoglobus fulgidus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.7.74 | crystal structure of the enzyme in the apo form is solved to a 1.9 A resolution. The structure of IPCT is composed of a central seven-stranded mixed beta-sheet, of which six beta-strands are parallel, surrounded by six alpha-helices, a fold reminiscent of the dinucleotide-binding Rossmann fold | Archaeoglobus fulgidus |
2.7.7.74 | hanging-drop vapor diffusion method at 20°C, X-ray structure of the cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34). The structure of the enzyme in the apo form was solved to a 1.9 A resolution. The structure of CTP:inositol-1-phosphate cytidylyltransferase is composed of a central seven-stranded mixed beta-sheet, of which six beta-strands are parallel, surrounded by six alpha-helices, a fold reminiscent of the dinucleotide-binding Rossmann fold | Archaeoglobus fulgidus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.74 | 0.58 | - |
CTP | pH and temperature not specified in the publication | Archaeoglobus fulgidus | |
2.7.7.74 | 0.58 | - |
CTP | 90°C, pH 7.0, cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) | Archaeoglobus fulgidus | |
2.7.7.74 | 0.87 | - |
1D-myo-inositol 3-phosphate | pH and temperature not specified in the publication | Archaeoglobus fulgidus | |
2.7.7.74 | 0.87 | - |
1L-myo-inositol 1-phosphate | 90°C, pH 7.0, cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) | Archaeoglobus fulgidus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.74 | Mg2+ | presence of Mg2+ is required for activity, maximal activity is obtained with 20 mM Mg2+ | Archaeoglobus fulgidus | |
2.7.7.74 | Mg2+ | required, maximal activity at 20 mM MgCl | Archaeoglobus fulgidus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.74 | CTP + 1L-myo-inositol 1-phosphate | Archaeoglobus fulgidus | the enzyme is involved in biosynthesis of the solute bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | diphosphate + CDP-1L-myo-inositol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.74 | Archaeoglobus fulgidus | O29976 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.74 | the cytoplasmic domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) is produced in Escherichia coli | Archaeoglobus fulgidus |
2.7.7.74 | using metal affinity chromatography and gel filtration | Archaeoglobus fulgidus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.74 | 62.9 | - |
90°C, pH 7.0, cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) | Archaeoglobus fulgidus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.74 | CTP + 1D-myo-inositol 3-phosphate | - |
Archaeoglobus fulgidus | diphosphate + CDP-L-myo-inositol | - |
? | |
2.7.7.74 | CTP + 1L-myo-inositol 1-phosphate | - |
Archaeoglobus fulgidus | diphosphate + CDP-1L-myo-inositol | - |
? | |
2.7.7.74 | CTP + 1L-myo-inositol 1-phosphate | the enzyme is involved in biosynthesis of the solute bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | Archaeoglobus fulgidus | diphosphate + CDP-1L-myo-inositol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.74 | dimer | - |
Archaeoglobus fulgidus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.74 | CTP:inositol-1-phosphate cytidylyltransferase | - |
Archaeoglobus fulgidus |
2.7.7.74 | IPCT | - |
Archaeoglobus fulgidus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.74 | 90 | 95 | - |
Archaeoglobus fulgidus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.74 | 60 | 100 | activity of the enzyme is undetectable at temperatures below 60°C, and maximal activity is reached between 90 and 95°C, at 100°C about 25% of maximal activity | Archaeoglobus fulgidus |
2.7.7.74 | 90 | 95 | maximal activity | Archaeoglobus fulgidus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.74 | 6.5 | 7.5 | - |
Archaeoglobus fulgidus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.74 | 5.5 | 8.5 | pH 5.5: about 30% of maximal activity, pH 8.5: about 65% of maximal activity | Archaeoglobus fulgidus |
2.7.7.74 | 6.5 | 7.5 | maximal activity | Archaeoglobus fulgidus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.7.74 | physiological function | the enzyme is involved in biosynthesis of the solute bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments | Archaeoglobus fulgidus |