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Literature summary extracted from

  • Brito, J.A.; Borges, N.; Vonrhein, C.; Santos, H.; Archer, M.
    Crystal structure of Archaeoglobus fulgidus CTP:inositol-1-phosphate cytidylyltransferase, a key enzyme for di-myo-inositol-phosphate synthesis in (hyper)thermophiles (2011), J. Bacteriol., 193, 2177-2185.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.74 cytoplasmic domain of IPCT is expressed in Escherichia coli as a His-tagged fusion protein Archaeoglobus fulgidus
2.7.7.74 the cytoplasmic domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) is produced in Escherichia coli Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.74 crystal structure of the enzyme in the apo form is solved to a 1.9 A resolution. The structure of IPCT is composed of a central seven-stranded mixed beta-sheet, of which six beta-strands are parallel, surrounded by six alpha-helices, a fold reminiscent of the dinucleotide-binding Rossmann fold Archaeoglobus fulgidus
2.7.7.74 hanging-drop vapor diffusion method at 20°C, X-ray structure of the cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34). The structure of the enzyme in the apo form was solved to a 1.9 A resolution. The structure of CTP:inositol-1-phosphate cytidylyltransferase is composed of a central seven-stranded mixed beta-sheet, of which six beta-strands are parallel, surrounded by six alpha-helices, a fold reminiscent of the dinucleotide-binding Rossmann fold Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.74 0.58
-
CTP pH and temperature not specified in the publication Archaeoglobus fulgidus
2.7.7.74 0.58
-
CTP 90°C, pH 7.0, cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) Archaeoglobus fulgidus
2.7.7.74 0.87
-
1D-myo-inositol 3-phosphate pH and temperature not specified in the publication Archaeoglobus fulgidus
2.7.7.74 0.87
-
1L-myo-inositol 1-phosphate 90°C, pH 7.0, cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.74 Mg2+ presence of Mg2+ is required for activity, maximal activity is obtained with 20 mM Mg2+ Archaeoglobus fulgidus
2.7.7.74 Mg2+ required, maximal activity at 20 mM MgCl Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.74 CTP + 1L-myo-inositol 1-phosphate Archaeoglobus fulgidus the enzyme is involved in biosynthesis of the solute bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments diphosphate + CDP-1L-myo-inositol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.74 Archaeoglobus fulgidus O29976
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.74 the cytoplasmic domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) is produced in Escherichia coli Archaeoglobus fulgidus
2.7.7.74 using metal affinity chromatography and gel filtration Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.74 62.9
-
90°C, pH 7.0, cytoplasmic CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme (EC 2.7.7.74/EC 2.7.8.34) Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.74 CTP + 1D-myo-inositol 3-phosphate
-
Archaeoglobus fulgidus diphosphate + CDP-L-myo-inositol
-
?
2.7.7.74 CTP + 1L-myo-inositol 1-phosphate
-
Archaeoglobus fulgidus diphosphate + CDP-1L-myo-inositol
-
?
2.7.7.74 CTP + 1L-myo-inositol 1-phosphate the enzyme is involved in biosynthesis of the solute bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments Archaeoglobus fulgidus diphosphate + CDP-1L-myo-inositol
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.74 dimer
-
Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.74 CTP:inositol-1-phosphate cytidylyltransferase
-
Archaeoglobus fulgidus
2.7.7.74 IPCT
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.74 90 95
-
Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.74 60 100 activity of the enzyme is undetectable at temperatures below 60°C, and maximal activity is reached between 90 and 95°C, at 100°C about 25% of maximal activity Archaeoglobus fulgidus
2.7.7.74 90 95 maximal activity Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.74 6.5 7.5
-
Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.74 5.5 8.5 pH 5.5: about 30% of maximal activity, pH 8.5: about 65% of maximal activity Archaeoglobus fulgidus
2.7.7.74 6.5 7.5 maximal activity Archaeoglobus fulgidus

General Information

EC Number General Information Comment Organism
2.7.7.74 physiological function the enzyme is involved in biosynthesis of the solute bis(1L-myo-inositol) 1,3'-phosphate, a widespread organic solute in microorganisms adapted to hot environments Archaeoglobus fulgidus