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Literature summary extracted from

  • Alvey, R.M.; Biswas, A.; Schluchter, W.M.; Bryant, D.A.
    Effects of modified phycobilin biosynthesis in the cyanobacterium Synechococcus sp. strain PCC 7002 (2011), J. Bacteriol., 193, 1663-1671.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.4 overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin, the cells show a phenotype only slightly less pigmented and blue-green than the wild-type, the strain producing phycobiliproteins carrying phytochromobilin grow much more slowly at low light intensity. Transformant colonies in which pcyA is inactivated in the HY2 overexpression background does not develop a chlorotic appearance, and segregation of the mutant and wild-type alleles is rapidly achieved Arabidopsis thaliana
1.3.7.6 gene pebAB, overexpression in Synechococcus sp. strain PCC 7002, from endogenous plasmid pAQ1 under the control of the Synechocystis sp. strain PCC 6803 cpcBA promoter, leads to overproduction of phytochromobilin. Colonies of Synechococcus sp. strain PCC 7002 transformed with the pebAB overexpression cassette mostly exhibit a reddish-brown phenotype Synechococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.3.7.5 additional information insertional inactivation of pcyA is not possible in wild-type Synechococcus sp. strain PCC 7002 due to lethality of the mutant, but in a variant heterologously expressing HY2 from Arabidopsis thaliana encoding a phytochromobilin:ferredoxin oxidoreductase Synechococcus sp.
1.3.7.6 additional information because PcyA, EC 1.3.7.5, and PebA utilize the same substrate, biliverdin IXalpha, severe overexpression of pebA can limit the availability of phycocyanobilin, which appears to be required for viability when cells are grown in continuous light, phenotype, overview Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.4 biliverdin IXalpha + reduced ferredoxin Arabidopsis thaliana
-
 (3Z)-phytochromobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin Synechococcus sp.
-
 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin Synechococcus sp. PCC 7335
-
 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin Synechococcus sp.
-
 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin Synechococcus sp. PCC 7335
-
 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Synechococcus sp.
-
 biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Synechococcus sp. PCC 7335
-
 biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.4 Arabidopsis thaliana
-
-
-
1.3.7.5 Synechococcus sp.
-
-
-
1.3.7.6 Synechococcus sp.
-
 gene pebAB
-
1.3.7.6 Synechococcus sp. PCC 7335
-
 gene pebAB
-
1.14.15.20 Synechococcus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.4 biliverdin IXalpha + reduced ferredoxin
-
 Arabidopsis thaliana (3Z)-phytochromobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin
-
 Synechococcus sp. (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin phycocyanobilin:ferredoxin oxidoreductase uses four electrons from reduced ferredoxin to synthesize phycocyanobilin by regiospecific reduction of the exo vinyl group of ring D and the endo vinyl group of ring A of biliverdin IXalpha Synechococcus sp. (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin
-
 Synechococcus sp. PCC 7335 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin phycocyanobilin:ferredoxin oxidoreductase uses four electrons from reduced ferredoxin to synthesize phycocyanobilin by regiospecific reduction of the exo vinyl group of ring D and the endo vinyl group of ring A of biliverdin IXalpha Synechococcus sp. PCC 7335 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin
-
 Synechococcus sp. (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin (3Z)-phycoerythrobilin is synthesized from biliverdin IXalpha by the sequential actions of two reductases, PebA and PebB Synechococcus sp. (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin
-
 Synechococcus sp. PCC 7335 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin (3Z)-phycoerythrobilin is synthesized from biliverdin IXalpha by the sequential actions of two reductases, PebA and PebB Synechococcus sp. PCC 7335 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Synechococcus sp. biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.20 protoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Synechococcus sp. PCC 7335 biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.4 HY2
-
 Arabidopsis thaliana
1.3.7.5 PCB:ferredoxin oxidoreductase
-
 Synechococcus sp.
1.3.7.5 PcyA
-
 Synechococcus sp.
1.3.7.5 phycocyanobilin synthase
-
 Synechococcus sp.
1.3.7.6 PebA
-
 Synechococcus sp.
1.3.7.6 PebB
-
 Synechococcus sp.
1.14.15.20 heme oxygenase
-
 Synechococcus sp.
1.14.15.20 Ho1
-
 Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.4 Ferredoxin
-
 Arabidopsis thaliana
1.3.7.5 Ferredoxin
-
 Synechococcus sp.
1.3.7.6 Ferredoxin
-
 Synechococcus sp.

General Information

EC Number General Information Comment Organism
1.3.7.4 evolution synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, overview Arabidopsis thaliana
1.3.7.5 evolution synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, ooverview Synechococcus sp.
1.3.7.5 malfunction because PcyA and PebA, EC 1.3.7.6/1.3.7.3, utilize the same substrate, biliverdin IXalpha, severe overexpression of pebA can limit the availability of phycocyanobilin, which appears to be required for viability when cells are grown in continuous light Synechococcus sp.
1.3.7.5 physiological function the pcyA gene of Synechococcus sp. strain PCC 7002 is essential Synechococcus sp.
1.3.7.6 evolution synthesis of linear tetrapyrrole chromophores in cyanobacteria, algae, and plants, ooverview Synechococcus sp.