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Literature summary extracted from

  • Yang, X.; Ma, K.
    Characterization of a thioredoxin-thioredoxin reductase system from the hyperthermophilic bacterium Thermotoga maritima (2010), J. Bacteriol., 192, 1370-1376.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.073
-
 NADH at pH 9.5 and 80°C Thermotoga maritima
1.8.1.9 0.089
-
 benzyl viologen at pH 9.5 and 80°C Thermotoga maritima
1.8.1.9 0.78
-
 NADPH at pH 6.5 and 80°C Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 31000
-
 2 * 31000, SDS-PAGE Thermotoga maritima
1.8.1.9 67000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 additional information Thermotoga maritima the enzyme cannot use thioredoxin from Spirulina as an electron acceptor ?
-
 ?
1.8.1.9 additional information Thermotoga maritima DSM 3109 the enzyme cannot use thioredoxin from Spirulina as an electron acceptor ?
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+ Thermotoga maritima
-
 thioredoxin + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+ Thermotoga maritima DSM 3109
-
 thioredoxin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Thermotoga maritima
-
-
-
1.8.1.9 Thermotoga maritima DSM 3109
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+
-
 Thermotoga maritima 5'-thionitrobenzoic acid + NAD+
-
 ?
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+
-
 Thermotoga maritima DSM 3109 5'-thionitrobenzoic acid + NAD+
-
 ?
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Thermotoga maritima 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Thermotoga maritima DSM 3109 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 benzyl viologen + NADH + H+
-
 Thermotoga maritima ?
-
 ?
1.8.1.9 benzyl viologen + NADH + H+
-
 Thermotoga maritima DSM 3109 ?
-
 ?
1.8.1.9 benzyl viologen + NADPH + H+
-
 Thermotoga maritima ?
-
 ?
1.8.1.9 additional information the enzyme cannot use thioredoxin from Spirulina as an electron acceptor Thermotoga maritima ?
-
 ?
1.8.1.9 additional information the enzyme cannot use thioredoxin from Spirulina as an electron acceptor Thermotoga maritima DSM 3109 ?
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Thermotoga maritima thioredoxin + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Thermotoga maritima DSM 3109 thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 homodimer 2 * 31000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 L-TR
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.9 80 95 enzyme activity increases along with the rise of temperature up to 95°C, and more than 60% of the activity remains after incubation for 28 h at 80°C and 50% remains after 9 h at 95°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.9 6.5
-
 with NADPH as cosubstrate Thermotoga maritima
1.8.1.9 9.5
-
 with NADH as cosubstrate Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD 1.88 mol of FAD per mol native enzyme Thermotoga maritima
1.8.1.9 NADH the enzyme shows a preference for NADH over NADPH Thermotoga maritima
1.8.1.9 NADPH
-
 Thermotoga maritima