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Literature summary extracted from

  • Friedmann, S.; Steindorf, A.; Alber, B.E.; Fuchs, G.
    Properties of succinyl-coenzyme A:L-malate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus (2006), J. Bacteriol., 188, 2646-2655.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.22 heterologously expressed in Escherichia coli Chloroflexus aurantiacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.22 Borohydride no inactivation by 0.1 mM borohydride is recorded in the test with and without succinyl-CoA, and nearly 85% of the activity is retained when the enzyme is preincubated with 10 mM hydroxylamine for 10 min in the presence of 2 mM succinyl-CoA. This suggests that no reactive enzyme-CoA intermediate is present. Inactivation with 10 mM borohydride decreases activity to 28% in the presence of 2 mM succinyl-CoA Chloroflexus aurantiacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.22 0.5
-
 succinyl-CoA 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus
2.8.3.22 1.1
-
 (S)-citramalate 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus
2.8.3.22 1.3
-
 (S)-malate 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.22 6000
-
 x * 6000 + x * 44000, (alphabeta)n complex, SDS-PAGE Chloroflexus aurantiacus
2.8.3.22 44000
-
 x * 6000 + x * 44000, (alphabeta)n complex, SDS-PAGE Chloroflexus aurantiacus
2.8.3.22 550000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.22 succinyl-CoA + (S)-malate Chloroflexus aurantiacus the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation succinate + (S)-malyl-CoA
-
 r
2.8.3.22 succinyl-CoA + (S)-malate Chloroflexus aurantiacus DSM Z 636 the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation succinate + (S)-malyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.22 Chloroflexus aurantiacus
-
-
-
2.8.3.22 Chloroflexus aurantiacus DSM Z 636
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.22
-
 Chloroflexus aurantiacus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.3.22 succinyl-CoA + (S)-citramalate = succinate + (S)-citramalyl-CoA (2) Chloroflexus aurantiacus
a
2.8.3.22 succinyl-CoA + (S)-malate = succinate + (S)-malyl-CoA (1) Chloroflexus aurantiacus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.22 7.5
-
 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus

Storage Stability

EC Number Storage Stability Organism
2.8.3.22 4°C, Tris buffer, pH 7, containing 100 mM KCl, 1 week, stable Chloroflexus aurantiacus
2.8.3.22 frozen, stable for several weeks with 10% glycerol (v/v) Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.22 succinyl-CoA + (S)-citramalate 2fold higher activity than with (S)-malate Chloroflexus aurantiacus succinate + (S)-citramalyl-CoA
-
 ?
2.8.3.22 succinyl-CoA + (S)-citramalate 2fold higher activity than with (S)-malate Chloroflexus aurantiacus DSM Z 636 succinate + (S)-citramalyl-CoA
-
 ?
2.8.3.22 succinyl-CoA + (S)-malate the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus succinate + (S)-malyl-CoA
-
 r
2.8.3.22 succinyl-CoA + (S)-malate no activity with (R)-malate. Succinyl-CoA is the only CoA donor found so far which could not be substituted by propionyl-CoA, malonyl-CoA, or acetyl-CoA Chloroflexus aurantiacus succinate + (S)-malyl-CoA
-
 r
2.8.3.22 succinyl-CoA + (S)-malate the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus DSM Z 636 succinate + (S)-malyl-CoA
-
 r
2.8.3.22 succinyl-CoA + (S)-malate no activity with (R)-malate. Succinyl-CoA is the only CoA donor found so far which could not be substituted by propionyl-CoA, malonyl-CoA, or acetyl-CoA Chloroflexus aurantiacus DSM Z 636 succinate + (S)-malyl-CoA
-
 r
2.8.3.22 succinyl-CoA + itaconate 12% of the activity compared to (S)-malate Chloroflexus aurantiacus succinate + ?
-
 ?
2.8.3.22 succinyl-CoA + itaconate 12% of the activity compared to (S)-malate Chloroflexus aurantiacus DSM Z 636 succinate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.22 ? x * 6000 + x * 44000, (alphabeta)n complex, SDS-PAGE Chloroflexus aurantiacus

Synonyms

EC Number Synonyms Comment Organism
2.8.3.22 SmtAB
-
 Chloroflexus aurantiacus
2.8.3.22 succinyl-CoA:L-malate coenzyme A transferase
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.22 55
-
 assay at Chloroflexus aurantiacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.3.22 11.4
-
 (S)-malate 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus
2.8.3.22 11.4
-
 succinyl-CoA 55°C, pH not specified in the publication, recombinant enzyme Chloroflexus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.22 6.5
-
 assay at Chloroflexus aurantiacus

General Information

EC Number General Information Comment Organism
2.8.3.22 physiological function the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation Chloroflexus aurantiacus