EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.15 | overproduced as an N-terminal His6-tagged fusion protein | Streptomyces globisporus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.15 | 0.742 | - |
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] | pH 6.0, 25°C | Streptomyces globisporus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.15 | 63200 | - |
x * 63200, calculated from sequence, SDS-PAGE | Streptomyces globisporus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.15 | S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | Streptomyces globisporus | the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027. FADH2 is supplied by the C-1027 pathway-specific flavin reductase SgcE6 | S-((3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.15 | Streptomyces globisporus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.15 | - |
Streptomyces globisporus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.15 | additional information | no activity with [peptidyl-carrier-protein SgcC2]-beta-tyrosyl thioester and [peptidyl-carrier-protein SgcC2]-(S)-3-hydroxy-beta-tyrosyl thioester | Streptomyces globisporus | ? | - |
? | |
1.14.14.15 | S-((3S)-3-amino-3-(3-bromo-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | - |
Streptomyces globisporus | S-((3S)-3-amino-3-(3-bromo-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? | |
1.14.14.15 | S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | - |
Streptomyces globisporus | S-((3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? | |
1.14.14.15 | S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027. FADH2 is supplied by the C-1027 pathway-specific flavin reductase SgcE6 | Streptomyces globisporus | S-((3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? | |
1.14.14.15 | S-((3S)-3-amino-3-(3-fluoro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | - |
Streptomyces globisporus | S-((3S)-3-amino-3-(3-fluoro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? | |
1.14.14.15 | S-((3S)-3-amino-3-(3-iodo-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | - |
Streptomyces globisporus | S-((3S)-3-amino-3-(3-iodo-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? | |
1.14.14.15 | S-((3S)-3-amino-3-(3-methyl-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | - |
Streptomyces globisporus | S-((3S)-3-amino-3-(3-methyl-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.15 | ? | x * 63200, calculated from sequence, SDS-PAGE | Streptomyces globisporus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.15 | SgcC | - |
Streptomyces globisporus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.15 | 25 | - |
assay at | Streptomyces globisporus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.15 | 0.023 | - |
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] | pH 6.0, 25°C | Streptomyces globisporus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.15 | 6 | - |
- |
Streptomyces globisporus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.15 | 5.5 | 7 | pH 5.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity | Streptomyces globisporus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.15 | physiological function | the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 | Streptomyces globisporus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.15 | 0.031 | - |
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] | pH 6.0, 25°C | Streptomyces globisporus |