EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.43 | expressed in Escherichia coli | Streptomyces carzinostaticus |
6.2.1.43 | expression in Escherichia coli | Streptomyces carzinostaticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.43 | 0.00025 | - |
2-hydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.00025 | - |
2-hydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.00059 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.00059 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0011 | - |
2-hydroxy-5,7-dimethoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0011 | - |
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0023 | - |
2-hydroxy-7-methoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0023 | - |
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0031 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0031 | - |
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0034 | - |
2-hydroxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.0034 | - |
2-hydroxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.018 | - |
1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.018 | - |
Naphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | Streptomyces carzinostaticus | 100% activity | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | Streptomyces carzinostaticus | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | Streptomyces carzinostaticus ATCC 15944 | 100% activity | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | Streptomyces carzinostaticus ATCC 15944 | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.43 | Streptomyces carzinostaticus | - |
- |
- |
6.2.1.43 | Streptomyces carzinostaticus ATCC 15944 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.43 | - |
Streptomyces carzinostaticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.43 | ATP + 1-naphthoate + CoA | kcat/Km is 0.6% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA | kcat/Km is 47% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA | kcat/Km is 47% compared to the wild-type value | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid + CoA | 47% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-1-naphthoate + CoA | kcat/Km is 1.3% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-1-naphthoate + CoA | kcat/Km is 1.3% compared to the wild-type value | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-5,7-dimethoxy-1-naphthoate + CoA | kcat/Km is 35% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-5,7-dimethoxy-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid + CoA | 35% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-5-methyl-1-naphthoate + CoA | kcat/Km is 50% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-5-methylnaphthalene-1-carboxylic acid + CoA | 50% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-1-naphthoate + CoA | kcat/Km is 5% compared to the wild-type value | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | - |
Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | 100% activity | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | - |
Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | 100% activity | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus ATCC 15944 | AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA | - |
? | |
6.2.1.43 | ATP + 2-hydroxy-7-methoxynaphthalene-1-carboxylic acid + CoA | 4.8% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
6.2.1.43 | ATP + 2-hydroxynaphthalene-1-carboxylic acid + CoA | 1.3% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? | |
6.2.1.43 | ATP + naphthalene-1-carboxylic acid + CoA | 0.58% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | AMP + diphosphate + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.43 | naphthoic acid/coenzyme A ligase | - |
Streptomyces carzinostaticus |
6.2.1.43 | naphthoyl CoA ligase | - |
Streptomyces carzinostaticus |
6.2.1.43 | NcsB2 | - |
Streptomyces carzinostaticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.43 | 0.047 | - |
2-hydroxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.05 | - |
2-hydroxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.11 | - |
Naphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.11 | - |
1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.118 | - |
2-hydroxy-7-methoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.12 | - |
2-hydroxy-7-methoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.135 | - |
2-hydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.135 | - |
2-hydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.42 | - |
2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.42 | - |
2-hydroxy-5,7-dimethoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 0.63 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 0.63 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 1.55 | - |
2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid | pH and temperature not specified in the publication | Streptomyces carzinostaticus | |
6.2.1.43 | 1.55 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.2.1.43 | physiological function | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.43 | 13.8 | - |
2-hydroxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 51.3 | - |
2-hydroxy-7-methoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 382 | - |
2-hydroxy-5,7-dimethoxy-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 500 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 540 | - |
2-hydroxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus | |
6.2.1.43 | 1068 | - |
2-hydroxy-7-methoxy-5-methyl-1-naphthoate | pH 8.0, 24°C | Streptomyces carzinostaticus |