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Literature summary extracted from

  • Leimkuhler, C.; Fridman, M.; Lupoli, T.; Walker, S.; Walsh, C.T.; Kahne, D.
    Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A (2007), J. Am. Chem. Soc., 129, 10546-10550.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.326 AKnT protein the optimal ratio of AknS to AknT is 1:3 Streptomyces galilaeus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.326 0.005
-
 aklavinone in the presence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
2.4.1.326 0.013
-
 aklavinone in the absence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
2.4.1.326 0.28
-
 dTDP-beta-L-rhodosamine in the presence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
2.4.1.326 0.349
-
 dTDP-beta-L-rhodosamine in the absence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B46 dTDP-2-deoxy-beta-L-fucose + 4-O-(alpha-L-rhodosaminyl)aklavinone Streptomyces galilaeus
-
 dTDP + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone
-
 ?
2.4.1.326 dTDP-beta-L-rhodosamine + aklavinone Streptomyces galilaeus
-
 dTDP + rhodosaminyl-aklavinone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B46 Streptomyces galilaeus
-
-
-
2.4.1.326 Streptomyces galilaeus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B46 dTDP-2-deoxy-alpha-L-fucose + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-[[4-O-alpha-L-2-deoxyfucosyl-(4-O-2-deoxy-alpha-L-fucosyl)]alpha-L-rhodosaminyl]aklavinone
-
 ?
2.4.1.B46 dTDP-2-deoxy-beta-L-fucose + 4-O-(alpha-L-rhodosaminyl)aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone
-
 ?
2.4.1.B46 additional information the enzyme does not transfer TDP-L-rhodosamine Streptomyces galilaeus ?
-
 ?
2.4.1.326 dTDP-beta-L-rhodosamine + aklavinone
-
 Streptomyces galilaeus dTDP + aclacinomycin T
-
 ?
2.4.1.326 dTDP-beta-L-rhodosamine + aklavinone
-
 Streptomyces galilaeus dTDP + rhodosaminyl-aklavinone
-
 ?
2.4.1.326 dTDP-L-2-deoxyfucose + aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone
-
 ?
2.4.1.326 dTDP-L-daunosamine + aklavinone
-
 Streptomyces galilaeus dTDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.326 AknS/AknT
-
 Streptomyces galilaeus
2.4.1.326 Gtf
-
 Streptomyces galilaeus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B46 25
-
 assay at Streptomyces galilaeus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.326 0.0083
-
 dTDP-beta-L-rhodosamine in the absence of activator protein AknT, at pH 7.5 and 25°C Streptomyces galilaeus
2.4.1.326 0.16
-
 dTDP-beta-L-rhodosamine at pH 7.5 and 25°C Streptomyces galilaeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B46 7.5
-
 assay at Streptomyces galilaeus