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Literature summary extracted from

  • Wojcieszynska, D.; Hupert-Kocurek, K.; Gren, I.; Guzik, U.
    High activity catechol 2,3-dioxygenase from the cresols - degrading Stenotrophomonas maltophilia strain KB2 (2011), Int. Biodeter. Biodegrad., 65, 853-858.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 DNA and amino acid sequence determination and analysis Stenotrophomonas maltophilia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 Fe2+ Fe2þ ion binding is facilitated via four ligands: two histidine residues, one glutamate residue and one molecule of water, overview Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 2-methylphenol + O2 Stenotrophomonas maltophilia
-
 ?
-
 ?
1.13.11.2 2-methylphenol + O2 Stenotrophomonas maltophilia KB2
-
 ?
-
 ?
1.13.11.2 3-methylphenol + O2 Stenotrophomonas maltophilia
-
 ?
-
 ?
1.13.11.2 3-methylphenol + O2 Stenotrophomonas maltophilia KB2
-
 ?
-
 ?
1.13.11.2 4-Methylphenol + O2 Stenotrophomonas maltophilia
-
 ?
-
 ?
1.13.11.2 4-Methylphenol + O2 Stenotrophomonas maltophilia KB2
-
 ?
-
 ?
1.13.11.2 catechol + O2 Stenotrophomonas maltophilia
-
 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2 Stenotrophomonas maltophilia KB2
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Stenotrophomonas maltophilia A7LB47
-
-
1.13.11.2 Stenotrophomonas maltophilia KB2 A7LB47
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 2-methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 2-methylphenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 3-methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 3-methylphenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 4-Methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 4-Methylphenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 catechol + O2
-
 Stenotrophomonas maltophilia 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Stenotrophomonas maltophilia KB2 2-hydroxymuconate-6-semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 More the secondary structure shows presence of externally located alpha-helices and internally located beta-sheets, enzyme molecular modeling, overview Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 30
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.2 35
-
 loss of 17.4% activity Stenotrophomonas maltophilia
1.13.11.2 35 40 rapid decline in activity Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 7.6
-
-
 Stenotrophomonas maltophilia

Expression

EC Number Organism Comment Expression
1.13.11.2 Stenotrophomonas maltophilia the enzyme in strain KB2 is induced by growth on methylphenols up

General Information

EC Number General Information Comment Organism
1.13.11.2 evolution the enzyme belongs to the single-ring substrate subfamily of the extradiol dioxygenase Stenotrophomonas maltophilia