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Literature summary extracted from

  • Pandeeti, E.V.; Siddavattam, D.
    Purification and characterization of catechol 1,2-dioxygenase from Acinetobacter sp. DS002 and cloning, sequencing of partial catA gene (2011), Indian J. Microbiol., 51, 312-318.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 gene catA, DNA and amino acid sequence determination and analysis Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 66000
-
 2 * 66000, SDS-PAGE Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Acinetobacter sp.
-
 cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 Acinetobacter sp. DS002
-
 cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Acinetobacter sp.
-
 gene catA
-
1.13.11.1 Acinetobacter sp. DS002
-
 gene catA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 native enzyme to homogeneity from cell supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, folloed by gel filtration Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 benzene-1,2,4-triol + O2 16% of the activity with catechol Acinetobacter sp. ?
-
 ?
1.13.11.1 benzene-1,2,4-triol + O2 16% of the activity with catechol Acinetobacter sp. DS002 ?
-
 ?
1.13.11.1 catechol + O2
-
 Acinetobacter sp. cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 best substrate Acinetobacter sp. cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Acinetobacter sp. DS002 cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 best substrate Acinetobacter sp. DS002 cis,cis-muconate
-
 ?
1.13.11.1 additional information poor or no activity with 3-methylcatechol, 4-methylcatechol, and 4-nitrocatechol Acinetobacter sp. ?
-
 ?
1.13.11.1 additional information poor or no activity with 3-methylcatechol, 4-methylcatechol, and 4-nitrocatechol Acinetobacter sp. DS002 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 homodimer 2 * 66000, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C12O
-
 Acinetobacter sp.

General Information

EC Number General Information Comment Organism
1.13.11.1 physiological function catechol 1,2-dioxygenase is the key enzyme in ortho pathway of catechol degradation Acinetobacter sp.