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Literature summary extracted from

  • Kumar, R.; Hooda, V.; Pundir, C.S.
    Purification and partial characterization of oxalate oxidase from leaves of forage Sorghum (Sorghum vulgare var. KH-105) seedlings (2011), Indian J. Biochem. Biophys., 48, 42-46.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.4 FAD 55.5% stimulation of activity at 0.5 mM Sorghum bicolor
1.2.3.4 FAD 55.5% stimulation of activity at 0.5 mM FAD Sorghum bicolor
1.2.3.4 FMN 45% stimulation of activity at 0.5 mM Sorghum bicolor
1.2.3.4 NAD+ 16.6% stimulation of activity at 0.5 mM FAD Sorghum bicolor
1.2.3.4 riboflavin 33.3% stimulation of activity at 0.5 mM Sorghum bicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 diethyldithiocarbamate strong inhibitor, 30.7% residual activity at 0.1 mM Sorghum bicolor
1.2.3.4 EDTA strong inhibitor, 61.5% residual activity at 0.1 mM Sorghum bicolor
1.2.3.4 H3PO4 76.9% residual activity at 0.1 mM Sorghum bicolor
1.2.3.4 additional information not influenced by FeSO4 Sorghum bicolor
1.2.3.4 Na2SO4 48.4% residual activity at 0.1 mM Sorghum bicolor
1.2.3.4 NaCN
-
 Sorghum bicolor
1.2.3.4 ZnSO4 61.3% residual activity at 0.1 mM Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 0.22
-
 oxalate at pH 4.5 and 37°C Sorghum bicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.4 Cu2+ 76% stimulation of activity at 0.5 mM Cu2+ Sorghum bicolor
1.2.3.4 additional information the enzyme is unaffected by 0.1 mM NaCl and Ca2+ Sorghum bicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.4 62000
-
 2 * 62000, SDS-PAGE Sorghum bicolor
1.2.3.4 124000
-
 gel filtration Sorghum bicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.3.4 oxalate + O2 + H+ Sorghum bicolor
-
 CO2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Sorghum bicolor
-
 variant KH-105
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.3.4 glycoprotein
-
 Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.4 leaf
-
 Sorghum bicolor
-
1.2.3.4 seedling
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalate + O2 + H+
-
 Sorghum bicolor CO2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.3.4 homodimer 2 * 62000, SDS-PAGE Sorghum bicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.4 37
-
-
 Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 4.5
-
-
 Sorghum bicolor