Literature summary extracted from

Kanao, T.; Matsumoto, C.; Shiraga, K.; Yoshida, K.; Takada, J.; Kamimura, K.
Recombinant tetrathionate hydrolase from Acidithiobacillus ferrooxidans requires exposure to acidic conditions for proper folding (2010), FEMS Microbiol. Lett., 309, 43-47.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.12.1.B1	expression in REscherichia coli	Acidithiobacillus ferrooxidans

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.12.1.B1	membrane	outer membrane	Acidithiobacillus ferrooxidans	16020	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.12.1.B1	Acidithiobacillus ferrooxidans	Q0KK37	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.12.1.B1	expression in Escherichia coli results in the formation of inclusion bodies of the protein in an inactive form. The recombinant protein can be successfully activated after an in vitro refolding treatment. The enzyme requires exposure to an acidicenvironment during protein folding for activation	Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.12.1.B1	21	-	recombinant protein, pH 3.0, 30°C	Acidithiobacillus ferrooxidans

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
3.12.1.B1	additional information	no cofactor required during the refolding process, no cofoactor required for activity	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)