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Literature summary extracted from

  • Nihira, T.; Saito, Y.; Nishimoto, M.; Kitaoka, M.; Igarashi, K.; Ohtsubo, K.; Nakai, H.
    Discovery of cellobionic acid phosphorylase in cellulolytic bacteria and fungi (2013), FEBS Lett., 587, 3556-3561.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.321 expressed in Escherichia coli Xanthomonas campestris pv. campestris
2.4.1.321 expressed in Escherichia coli Neurospora crassa
2.4.1.321 expression in Escherichia coli as a His-tagged fusion protein Neurospora crassa
2.4.1.321 expression in Escherichia coli as a His-tagged fusion protein Xanthomonas campestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate
-
Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.321 0.1
-
phosphate pH 7.0, 30°C Neurospora crassa
2.4.1.321 0.21
-
alpha-D-glucose 1-phosphate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 0.21
-
alpha-D-glucose 1-phosphate pH 6.0-6.5, 30°C Xanthomonas campestris pv. campestris
2.4.1.321 1
-
4-O-beta-D-glucopyranosyl-D-gluconate pH 7.0, 30°C Neurospora crassa
2.4.1.321 2
-
D-gluconate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 2
-
D-gluconate pH 6.0-6.5, 30°C Xanthomonas campestris pv. campestris
2.4.1.321 2.4
-
D-glucuronate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 2.4
-
D-glucuronate pH 6.0-6.5, 30°C Xanthomonas campestris pv. campestris
2.4.1.321 3.4
-
D-gluconate pH 6.5, 30°C Neurospora crassa
2.4.1.321 3.4
-
D-gluconate pH 6.0-6.5, 30°C Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.321 cytoplasm
-
Xanthomonas campestris pv. campestris 5737
-
2.4.1.321 cytoplasm
-
Neurospora crassa 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.321 84000
-
2 * 84000, SDS-PAGE Xanthomonas campestris
2.4.1.321 84000
-
2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence Xanthomonas campestris pv. campestris
2.4.1.321 87000
-
2 * 87000, SDS-PAGE Neurospora crassa
2.4.1.321 87000
-
2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence Neurospora crassa
2.4.1.321 89700
-
2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence Xanthomonas campestris pv. campestris
2.4.1.321 89700
-
2 * 89700, calculated from sequence Xanthomonas campestris
2.4.1.321 90769
-
2 * 90769, calculated from sequence Neurospora crassa
2.4.1.321 90796
-
2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence Neurospora crassa
2.4.1.321 171000
-
gel filtration Neurospora crassa
2.4.1.321 179000
-
gel filtration Xanthomonas campestris pv. campestris
2.4.1.321 179000
-
gel filtration Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate Neurospora crassa
-
alpha-D-glucose 1 phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate Xanthomonas campestris pv. campestris
-
alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate Neurospora crassa it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate Xanthomonas campestris it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate Neurospora crassa DSM 1257
-
alpha-D-glucose 1 phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate Neurospora crassa DSM 1257 it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate Xanthomonas campestris pv. campestris ATCC 33913
-
alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 alpha-D-glucose 1 phosphate + D-gluconate Neurospora crassa
-
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1 phosphate + D-gluconate Neurospora crassa DSM 1257
-
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-gluconate Xanthomonas campestris pv. campestris
-
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-gluconate Xanthomonas campestris pv. campestris ATCC 33913
-
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.321 Neurospora crassa Q7S0S2
-
-
2.4.1.321 Neurospora crassa DSM 1257 Q7S0S2
-
-
2.4.1.321 Xanthomonas campestris Q8P3J4
-
-
2.4.1.321 Xanthomonas campestris pv. campestris Q8P3J4
-
-
2.4.1.321 Xanthomonas campestris pv. campestris ATCC 33913 Q8P3J4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.321
-
Xanthomonas campestris pv. campestris
2.4.1.321
-
Neurospora crassa
2.4.1.321
-
Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
Neurospora crassa alpha-D-glucose 1 phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
Neurospora crassa DSM 1257 alpha-D-glucose 1 phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
Xanthomonas campestris pv. campestris alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively Neurospora crassa alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively Xanthomonas campestris alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate 4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bi–bi mechanism Neurospora crassa alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate 4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bi–bi mechanism Xanthomonas campestris alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively Neurospora crassa DSM 1257 alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate 4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bi–bi mechanism Neurospora crassa DSM 1257 alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
Xanthomonas campestris pv. campestris ATCC 33913 alpha-D-glucose 1-phosphate + D-gluconate
-
r
2.4.1.321 alpha-D-glucose 1 phosphate + D-gluconate
-
Neurospora crassa 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1 phosphate + D-gluconate
-
Neurospora crassa DSM 1257 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-gluconate
-
Xanthomonas campestris pv. campestris 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-gluconate
-
Neurospora crassa 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-gluconate
-
Xanthomonas campestris 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-gluconate
-
Neurospora crassa DSM 1257 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-gluconate
-
Xanthomonas campestris pv. campestris ATCC 33913 4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-glucuronate
-
Xanthomonas campestris pv. campestris 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
r
2.4.1.321 alpha-D-glucose 1-phosphate + D-glucuronate in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity Neurospora crassa 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
?
2.4.1.321 alpha-D-glucose 1-phosphate + D-glucuronate in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity Xanthomonas campestris 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
?
2.4.1.321 alpha-D-glucose 1-phosphate + D-glucuronate
-
Xanthomonas campestris pv. campestris ATCC 33913 4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.4.1.321 homodimer 2 * 84000, SDS-PAGE Xanthomonas campestris
2.4.1.321 homodimer 2 * 87000, SDS-PAGE Neurospora crassa
2.4.1.321 homodimer 2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence Xanthomonas campestris pv. campestris
2.4.1.321 homodimer 2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence Neurospora crassa
2.4.1.321 homodimer 2 * 89700, calculated from sequence Xanthomonas campestris
2.4.1.321 homodimer 2 * 90769, calculated from sequence Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase
-
Neurospora crassa
2.4.1.321 4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase
-
Xanthomonas campestris
2.4.1.321 NCU09425 locus name Neurospora crassa
2.4.1.321 XCC4077 locus name Xanthomonas campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.321 30
-
assay at Neurospora crassa
2.4.1.321 30
-
assay at Xanthomonas campestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.321 35
-
15 min, stable Neurospora crassa
2.4.1.321 35
-
15 min, stable Xanthomonas campestris
2.4.1.321 35
-
15 min stable up to Xanthomonas campestris pv. campestris
2.4.1.321 35
-
15 min stable up to Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.321 43
-
D-glucuronate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 43
-
D-glucuronate pH 6.0-6.5, 30°C Xanthomonas campestris pv. campestris
2.4.1.321 127
-
alpha-D-glucose 1-phosphate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 127
-
alpha-D-glucose 1-phosphate pH 6.0-6.5, 30°C Xanthomonas campestris pv. campestris
2.4.1.321 146
-
D-gluconate pH 6.5, 30°C Neurospora crassa
2.4.1.321 146
-
D-gluconate pH 6.0-6.5, 30°C Neurospora crassa
2.4.1.321 173
-
D-gluconate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 173
-
D-gluconate pH 6.0-6.5, 30°C Xanthomonas campestris pv. campestris
2.4.1.321 306
-
phosphate pH 7.0, 30°C Neurospora crassa
2.4.1.321 306
-
4-O-beta-D-glucopyranosyl-D-gluconate pH 7.0, 30°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.321 6
-
synthetic activity Xanthomonas campestris
2.4.1.321 6
-
synthetic reaction Xanthomonas campestris pv. campestris
2.4.1.321 6.5
-
synthetic activity Neurospora crassa
2.4.1.321 6.5
-
synthetic reaction Neurospora crassa
2.4.1.321 7
-
phosphorolytic reaction Xanthomonas campestris pv. campestris
2.4.1.321 7
-
phosphorolytic reaction Neurospora crassa
2.4.1.321 7
-
phosphorolytic activity Neurospora crassa
2.4.1.321 7
-
phosphorolytic activity Xanthomonas campestris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.321 5 10 4°C, 24 h, stable Xanthomonas campestris
2.4.1.321 5 10.5
-
Xanthomonas campestris pv. campestris
2.4.1.321 5.5 9
-
Neurospora crassa
2.4.1.321 5.5 9 4°C, 24 h, stable Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.321 7.3
-
4-O-beta-D-glucopyranosyl-D-gluconate pH 7.0, 30°C Neurospora crassa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.321 18
-
D-glucuronate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 87
-
D-gluconate pH 6.5, 30°C Xanthomonas campestris
2.4.1.321 605
-
alpha-D-glucose 1-phosphate pH 6.5, 30°C Xanthomonas campestris