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Literature summary extracted from

  • Agren, D.; Schnell, R.; Schneider, G.
    The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity (2009), FEBS Lett., 583, 330-336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.113 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.113
-
 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.113 DELTA319-323 the deletion mutant shows increased catalytic efficiency for O-phospho-L-serine and reduced catalytic efficiency for [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.113 O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?
2.5.1.113 O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis H37Rv
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.113 Mycobacterium tuberculosis P9WP53
-
-
2.5.1.113 Mycobacterium tuberculosis H37Rv P9WP53
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.113 Ni-NTA affinity column chromatography and Sephadex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.113 O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
 Mycobacterium tuberculosis [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?
2.5.1.113 O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
 Mycobacterium tuberculosis H37Rv [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.113 homodimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.113 CysM
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.113 0.857
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH deletion mutant DELTA319-323, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.5.1.113 1.34
-
 O-phospho-L-serine wild type enzyme, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.5.1.113 8.85
-
 O-phospho-L-serine deletion mutant DELTA319-323, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.5.1.113 146
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH wild type enzyme, at pH 7.5 and 37°C Mycobacterium tuberculosis