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Literature summary extracted from

  • Yang, C.A.; Cheng, C.H.; Liu, S.Y.; Lo, C.T.; Lee, J.W.; Peng, K.C.
    Identification of antibacterial mechanism of L-amino acid oxidase derived from Trichoderma harzianum ETS 323 (2011), FEBS J., 278, 3381-3394.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.2 medicine the antibacterial activity of the enzyme is similar to that of kanamycin Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 11.73
-
 L-phenylalanine at pH 7.0 and 40°C Trichoderma harzianum
1.4.3.2 12.01
-
 L-phenylalanine at pH 7.0 and 30°C Trichoderma harzianum
1.4.3.2 12.81
-
 L-phenylalanine at pH 7.0 and 20°C Trichoderma harzianum
1.4.3.2 13.52
-
 L-phenylalanine at pH 7.0 and 50°C Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Trichoderma harzianum
-
-
-
1.4.3.2 Trichoderma harzianum ETS 323
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2
-
 Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-phenylalanine + H2O + O2
-
 Trichoderma harzianum phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2
-
 Trichoderma harzianum ETS 323 phenylpyruvate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-amino acid oxidase
-
 Trichoderma harzianum
1.4.3.2 LAAO
-
 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 40
-
 at pH 7.0 Trichoderma harzianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.2 35 45 decrease of activity to 35-50% and 90-78% of maximal activity at 20-30°C and 45-50°C, respectively Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 40
-
 the enzyme rapidly becomes thermally denatured at temperatures above 40°C Trichoderma harzianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 0.008
-
 L-phenylalanine at pH 7.0 and 20°C Trichoderma harzianum
1.4.3.2 0.013
-
 L-phenylalanine at pH 7.0 and 30°C Trichoderma harzianum
1.4.3.2 0.02
-
 L-phenylalanine at pH 7.0 and 50°C Trichoderma harzianum
1.4.3.2 0.025
-
 L-phenylalanine at pH 7.0 and 40°C Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7
-
-
 Trichoderma harzianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.2 6 7.5
-
 Trichoderma harzianum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.2 4 9 the enzyme is stable at pH 6.0-8.0 (90% activity) and less stable at both pH 4.0-5.5 (less than 40% of maximal activity) and pH 9.0 (77% of maximal activity) Trichoderma harzianum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
 Trichoderma harzianum

General Information

EC Number General Information Comment Organism
1.4.3.2 physiological function the enzyme interacts with bacteria to cause membrane permeabilization. Reactive oxidative species accumulation triggers forms of cell damage, including lipid peroxidation and DNA strand breakage that results in bacterial growth inhibition Trichoderma harzianum