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Literature summary extracted from

  • Kagan, M.; Kivirand, K.; Rinken, T.
    Modulation of enzyme catalytic properties and biosensor calibration parameters with chlorides: Studies with glucose oxidase (2013), Enzyme Microb. Technol., 53, 278-282.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 analysis the enzyme is useful as biosensor for glucose detection Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.4 additional information modulation of calibration parameters of biosensors, in which glucose oxidase is used for biorecognition, in the presence of different chlorides by following the transient phase dynamics ofoxygen concentration with an oxygen optrode, mechanism, overview. the maximum calculated signal change was amplifiedfor about 20% in the presence of sodium and magnesium chlorides. The value of the kinetic parameter decreases along with the addition of salts and increases only at sodium chloride concentrations over 0.5 mM, MgCl2 causes a 1.3fold essential increase of the maximum signal change parameter A in a salt concentration, ranging from 0.1 to 0.4 M. AlCl3 inhibits the enzyme at 5 mM, and at higher salt concentrations over 0.1 M, the catalytic activity is completely inhibited Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.4 AlCl3 inhibits the enzyme at low concentrations of 5 mM, and inhibits it completely at higher salt concentrations over 0.1 M Aspergillus niger
1.1.3.4 Mg2+ total inhibition of the enzyme at a Mg2+ concentration of 1.9 M Aspergillus niger
1.1.3.4 Na+ the inhibiting effect of Na+ions is reduced at Na+concentrations over 0.5 M Aspergillus niger
1.1.3.4 NaNO3 a competitive inhibitor of glucose oxidase Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 commercial preparation
-
 Aspergillus niger
-

Synonyms

EC Number Synonyms Comment Organism
1.1.3.4 GOD
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 25
-
 assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.6
-
 assay at Aspergillus niger