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Literature summary extracted from

  • Flitsch, A.; Prasetyo, E.N.; Sygmund, C.; Ludwig, R.; Nyanhongo, G.S.; Guebitz, G.M.
    Cellulose oxidation and bleaching processes based on recombinant Myriococcum thermophilum cellobiose dehydrogenase (2013), Enzyme Microb. Technol., 52, 60-67.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.18 expressed in Pichia pastoris X-33 cells Thermothelomyces myriococcoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.035
-
 cellobiose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.085
-
 cellotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.09
-
 lactose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.71
-
 maltotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 6.75
-
 maltose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 387
-
 D-glucose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Thermothelomyces myriococcoides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 ammonium sulfate precipitation and phenyl Sepharose column chromatography Thermothelomyces myriococcoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 0.2
-
 culture supernatant, in 100 mM sodium acetate buffer, pH 5.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 3.1
-
 after 15.5fold purification, in 100 mM sodium acetate buffer, pH 5.0, at 30°C Thermothelomyces myriococcoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces myriococcoides cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces myriococcoides cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol indophenol weak substrate Thermothelomyces myriococcoides D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces myriococcoides 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Thermothelomyces myriococcoides 4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 maltotetraose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces myriococcoides ? + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 maltotriose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces myriococcoides ? + reduced 2,6-dichlorophenol indophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 Cdh
-
 Thermothelomyces myriococcoides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 0.315
-
 maltose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.48
-
 maltotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 2.7
-
 cellotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 4.11
-
 cellobiose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 4.68
-
 lactose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 4.8
-
 D-glucose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.18 0.012
-
 D-glucose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.047
-
 maltose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.676
-
 maltotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 32.12
-
 cellotetraose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 52
-
 lactose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 117.1
-
 cellobiose in 100 mM sodium acetate buffer, pH 4.0, at 30°C Thermothelomyces myriococcoides