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Literature summary extracted from

  • Pedruzzi, I.; da Silva, E.A.; Rodrigues, A.E.
    Production of lactobionic acid and sorbitol from lactose/fructose substrate using GFOR/GL enzymes from Zymomonas mobilis cells: a kinetic study (2011), Enzyme Microb. Technol., 49, 183-191.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.28 D-glucose + D-fructose Zymomonas mobilis at pH 6.4 and 39°C when 700 mM lactose/350 mM fructose pair substrate is used, the maximum possible conversion yield is attained after 24 h of operation D-glucono-1,5-lactone + D-sorbitol
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?
1.1.99.28 D-glucose + D-fructose Zymomonas mobilis ATCC 29191 at pH 6.4 and 39°C when 700 mM lactose/350 mM fructose pair substrate is used, the maximum possible conversion yield is attained after 24 h of operation D-glucono-1,5-lactone + D-sorbitol
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Organism

EC Number Organism UniProt Comment Textmining
1.1.99.28 Zymomonas mobilis
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-
-
1.1.99.28 Zymomonas mobilis ATCC 29191
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.28 D-glucose + D-fructose at pH 6.4 and 39°C when 700 mM lactose/350 mM fructose pair substrate is used, the maximum possible conversion yield is attained after 24 h of operation Zymomonas mobilis D-glucono-1,5-lactone + D-sorbitol
-
?
1.1.99.28 D-glucose + D-fructose at pH 6.4 and 39°C when 700 mM lactose/350 mM fructose pair substrate is used, the maximum possible conversion yield is attained after 24 h of operation Zymomonas mobilis ATCC 29191 D-glucono-1,5-lactone + D-sorbitol
-
?

Subunits

EC Number Subunits Comment Organism
1.1.99.28 tetramer
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Zymomonas mobilis

Synonyms

EC Number Synonyms Comment Organism
1.1.99.28 GFOR
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Zymomonas mobilis
1.1.99.28 glucose fructose oxidoreductase
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Zymomonas mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.28 NADP+ the enzyme is bound to NADP+ Zymomonas mobilis