Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Casaite, V.; Poviloniene, S.; Meskiene, R.; Rutkiene, R.; Meskys, R.
    Studies of dimethylglycine oxidase isoenzymes in Arthrobacter globiformis cells (2011), Curr. Microbiol., 62, 1267-1273.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.3.10 glycine betaine
-
 Arthrobacter globiformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.10 two dmg genes, dmg and dmg2, DNA and amino acid sequence determination, genetic organization, quantitative real-time pCR expression analysis Arthrobacter globiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.10 NaCl the specific activity of DMGO decreased when cells are grown in the medium with high NaCl concentration. High osmolarity inhibits the activity of the enzyme, but not in the case of the medium with glycine betaine as the sole carbon source Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.10 1.39
-
 N,N-dimethylglycine DMGO1, pH 9.0, temperature not specified in the publication Arthrobacter globiformis
1.5.3.10 1.86
-
 N,N-dimethylglycine DMGO2, pH 9.0, temperature not specified in the publication Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.10 N,N-dimethylglycine + H2O + O2 Arthrobacter globiformis
-
 sarcosine + formaldehyde + H2O2
-
 ?
1.5.3.10 N,N-dimethylglycine + H2O + O2 Arthrobacter globiformis NRRL B-2979
-
 sarcosine + formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.10 Arthrobacter globiformis
-
 two dmg genes, dmg and dmg2
-
1.5.3.10 Arthrobacter globiformis NRRL B-2979
-
 two dmg genes, dmg and dmg2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.10 culture condition:glycine betaine-grown cell
-
 Arthrobacter globiformis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.10 N,N-dimethylglycine + H2O + O2
-
 Arthrobacter globiformis sarcosine + formaldehyde + H2O2
-
 ?
1.5.3.10 N,N-dimethylglycine + H2O + O2
-
 Arthrobacter globiformis NRRL B-2979 sarcosine + formaldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.10 dimethylglycine dehydrogenase
-
 Arthrobacter globiformis
1.5.3.10 DMGO
-
 Arthrobacter globiformis
1.5.3.10 DMGO1
-
 Arthrobacter globiformis
1.5.3.10 DMGO2
-
 Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.10 30
-
 DMGO2 Arthrobacter globiformis
1.5.3.10 37
-
 DMGO1 Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.10 9.5
-
 DMGO1 and DMGO2 Arthrobacter globiformis

Expression

EC Number Organism Comment Expression
1.5.3.10 Arthrobacter globiformis glycine betaine induces the expression of the dmg genes by about 300fold at 5 mM up

General Information

EC Number General Information Comment Organism
1.5.3.10 metabolism in high-salinity media with 1 M NaCl, Arthrobacter globiformis accumulates 360 mM of glycine betaine when the main source of carbon is glucose and 180 mM glycine betaine when the sole carbon source is glycine betaine Arthrobacter globiformis