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Literature summary extracted from

  • Soma, H.; Furuya, R.; Kaneko, R.; Tsukamoto, A.; Sirasu, K.; Tanigawa, M.; Nagata, Y.
    D-Amino acid oxidase and presence of D-proline in Xenopus laevis (2013), Comp. Biochem. Physiol. B, 166, 165-171.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 benzoate moderate inhibition (49.7% residual activity at 1 mM) Xenopus laevis
1.4.3.3 Ca2+ 83.1% residual activity at 10 mM Xenopus laevis
1.4.3.3 iodoacetamide 89.8% residual activity at 1 mM Xenopus laevis
1.4.3.3 iodoacetate 81.6% residual activity at 1 mM Xenopus laevis
1.4.3.3 malate 78.6% residual activity at 10 mM Xenopus laevis
1.4.3.3 Mg2+ 84.9% residual activity at 10 mM Xenopus laevis
1.4.3.3 p-chloromercuribenzoate moderate inhibition (61.2% residual activity at 1 mM) Xenopus laevis
1.4.3.3 p-hydroxymercuribenzoate significant inhibition (6.2% residual activity at 1 mM) Xenopus laevis
1.4.3.3 succinate 90.7% residual activity at 1 mM Xenopus laevis
1.4.3.3 Tartrate 79.5% residual activity at 10 mM Xenopus laevis
1.4.3.3 Zn2+ 44% residual activity at 10 mM Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 39000
-
 x * 39000, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-amino acid + H2O + O2 Xenopus laevis
-
 2-oxocarboxylate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 ammonium sulfate precipitation, Toyopearl butyl column chromatography, and Q Sepharose column chromatography Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 adult
-
 Xenopus laevis
-
1.4.3.3 gut
-
 Xenopus laevis
-
1.4.3.3 heart lowest activity in heart Xenopus laevis
-
1.4.3.3 kidney highest activity in kidney Xenopus laevis
-
1.4.3.3 larva
-
 Xenopus laevis
-
1.4.3.3 liver
-
 Xenopus laevis
-
1.4.3.3 lung
-
 Xenopus laevis
-
1.4.3.3 stomach
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 0.0018
-
 cell-free extract from tadpoles, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C Xenopus laevis
1.4.3.3 0.0135
-
 cell-free extract from adults with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C Xenopus laevis
1.4.3.3 0.165
-
 enzyme from adults after 3563fold purification, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C Xenopus laevis
1.4.3.3 0.641
-
 enzyme from tadpoles after 3563fold purification, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 5.8% activity in tadpoles compared to D-proline Xenopus laevis pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-amino acid + H2O + O2
-
 Xenopus laevis 2-oxocarboxylate + NH3 + H2O2
-
 ?
1.4.3.3 D-aspartate + H2O + O2 1.0% activity in tadpoles compared to D-proline Xenopus laevis oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2 2.3% activity in tadpoles compared to D-proline Xenopus laevis 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2 7.6% activity in tadpoles compared to D-proline Xenopus laevis 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2 second best substrate Xenopus laevis phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2 1.1% activity in tadpoles compared to D-proline Xenopus laevis 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2 best substrate, 100% activity in tadpoles and adults Xenopus laevis 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2 7.6% activity in tadpoles compared to D-proline Xenopus laevis 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 ? x * 39000, SDS-PAGE Xenopus laevis

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Xenopus laevis
1.4.3.3 DAO
-
 Xenopus laevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.3 35 40
-
 Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 8.3 9
-
 Xenopus laevis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Xenopus laevis

Expression

EC Number Organism Comment Expression
1.4.3.3 Xenopus laevis enzyme activity is reduced to one-third in the adult down
1.4.3.3 Xenopus laevis enzyme activity is increased until the final tadpole stage up

General Information

EC Number General Information Comment Organism
1.4.3.3 physiological function D-amino acid oxidase functions in metamorphosis Xenopus laevis