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Literature summary extracted from

  • Schwab, T.; Sterner, R.
    Stabilization of a metabolic enzyme by library selection in Thermus thermophilus (2011), Chembiochem, 12, 1581-1588.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.18 D83G/F149S mutation leads to monomerization, apparent melting temperature is 9.5°C lower than the wild-type value Saccharolobus solfataricus
2.4.2.18 I36E/M47D mutation leads to monomerization, apparent melting temperature is 11.5°C lower than the wild-type value Saccharolobus solfataricus
2.4.2.18 I36E/M47D/D83G/F149S mutation leads to monomerization, apparent melting temperature is 21.4°C lower than the wild-type value. kcat/Km for anthranilate is 14.5fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 33fold higher than kcat/Km for wild-type enzyme Saccharolobus solfataricus
2.4.2.18 I36E/M47D/D83G/F149S/F193S mutation leads to monomerization, apparent melting temperature is 17.4°C lower than the wild-type value. kcat/Km for anthranilate is 52fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 31.3fold higher than kcat/Km for wild-type enzyme Saccharolobus solfataricus
2.4.2.18 I36E/M47D/D83G/F149S/I169T mutation leads to monomerization, apparent melting temperature is 20.6°C lower than the wild-type value. kcat/Km for anthranilate is 8.9fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 24.3fold higher than kcat/Km for wild-type enzyme Saccharolobus solfataricus
2.4.2.18 I36E/M47D/D83G/F149S/L320M mutation leads to monomerization, apparent melting temperature is 20.9°C lower than the wild-type value. kcat/Km for anthranilate is 11fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 31.3fold higher than kcat/Km for wild-type enzyme Saccharolobus solfataricus
2.4.2.18 I36E/M47D/D83G/N109S/F149S mutation leads to monomerization, apparent melting temperature is 20.5°C lower than the wild-type value. kcat/Km for anthranilate is 39fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 10fold higher than kcat/Km for wild-type enzyme Saccharolobus solfataricus
2.4.2.18 I36E/M47D/D83G/N109S/F149S/I169T/L320M/N324I mutation leads to monomerization, apparent melting temperature is 18.5°C lower than the wild-type value Saccharolobus solfataricus
2.4.2.18 I36E/M47D/T77I/D83G/F149S mutation leads to monomerization, apparent melting temperature is 13.3°C lower than the wild-type value. kcat/Km for anthranilate is 13.3fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 10fold higher than kcat/Km for wild-type enzyme Saccharolobus solfataricus
2.4.2.18 I36E/M47D/T77I/D83G/F149S/F193S mutation leads to monomerization, apparent melting temperature is 11.4°C lower than the wild-type value Saccharolobus solfataricus
2.4.2.18 I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M mutation leads to monomerization, apparent melting temperature is 9.1°C lower than the wild-type value. kcat/Km for anthranilate is 85.7fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 2.7fold lower than kcat/Km for wild-type enzyme Saccharolobus solfataricus
2.4.2.18 I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M mutation leads to monomerization, apparent melting temperature is 8.7°C lower than the wild-type value. kcat/Km for anthranilate is 209fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is higher than kcat/Km for wild-type enzyme Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.18 0.007
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.008
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.011
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.029
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.033
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.037
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.037
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.045
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.047
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.051
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.054
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.055
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.055
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.06
-
 anthranilate mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.062
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.082
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Saccharolobus solfataricus catalyzes the third step in tryptophan biosynthesis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Saccharolobus solfataricus P2 catalyzes the third step in tryptophan biosynthesis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.18 Saccharolobus solfataricus P50384
-
-
2.4.2.18 Saccharolobus solfataricus P2 P50384
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate catalyzes the third step in tryptophan biosynthesis Saccharolobus solfataricus N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus P2 N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate catalyzes the third step in tryptophan biosynthesis Saccharolobus solfataricus P2 N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.18 sAnPRT
-
 Saccharolobus solfataricus
2.4.2.18 SSO0890 locus name Saccharolobus solfataricus
2.4.2.18 strpD
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.18 37
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.18 0.046
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.046
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.78
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.78
-
 anthranilate mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 1.2
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 1.2
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 1.3
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 1.3
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 2.7
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 2.7
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 3.4
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 3.4
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 3.5
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 3.5
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 3.9
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 3.9
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.18 7.2
-
 assay at Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
2.4.2.18 physiological function catalyzes the third step in tryptophan biosynthesis Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.18 0.56
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 0.84
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 13
-
 anthranilate mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 23
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 24
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 24
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 25
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 56
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 72
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 72
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 76
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 92
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 330
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 360
-
 anthranilate mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 460
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C Saccharolobus solfataricus
2.4.2.18 540
-
 anthranilate mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C Saccharolobus solfataricus